Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression modification in lungs of rats exposed to carbon nanotube by inhalation [Mitsui]

ABSTRACT: Gene expression modification in lungs of rats exposed to carbon nanotube by inhalation [Mitsui]

PROVIDER: PRJNA672165 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression modification in lungs of rats exposed to carbon nanotube by inhalation [Mitsui]

Project description:In this work we study the pulmonary toxicological properties of Mitsui using molecular toxicological approache. For this, we exposed Sprague Dawley rats by inhalation. Lung samples have been collected up to 180 days after the end of exposure and transcriptomics analysis were performed.

2023-07-19 | GSE160175 | GEO

Gene expression modification in lungs of rats exposed to carbon black by inhalation

Project description:In this work we study the pulmonary toxicological properties of Printex using molecular toxicological approache. For this, we exposed Sprague Dawley rats by inhalation. Lung samples have been collected up to 180 days after the end of exposure and transcriptomics analysis were performed.

2023-07-19 | GSE160155 | GEO

Gene expression modification in lungs of rats exposed to carbon black by inhalation

Project description:Gene expression modification in lungs of rats exposed to carbon black by inhalation

| PRJNA672115 | ENA

Gene expression modification in lungs of rats exposed to TiO2 by inhalation

Project description:Gene expression modification in lungs of rats exposed to TiO2 by inhalation

| PRJNA671874 | ENA

Gene expression modification in lungs of rats exposed to TiO2 by inhalation

Project description:In this work we study the pulmonary toxicological properties of titanium dioxide using molecular toxicological approache. For this, we exposed Sprague Dawley rats by inhalation. Lung samples have been collected up to 180 days after the end of exposure and transcriptomics analysis were performed.

2023-07-19 | GSE160117 | GEO

Lungs of mice exposed to subtoxic doses of nano-titanium dioxide particles by inhalation

2010-12-13 | GSE19487 | GEO

Time-course of transcriptomic change in the lungs of F344 rats repeatedly exposed to a multiwalled carbon nanotube in a 2-year test

Project description:Despite intensive toxicological studies of carbon nanotubes (CNTs) over the last two decades, only a few studies have demonstrated their pulmonary carcinogenicities in chronic animal experiments, and the underlying molecular mechanisms are still unclear. To obtain molecular insights into CNT-induced lung carcinogenicity, we performed a transcriptomic analysis using a set of lung tissues collected from rats in a 2-year study, in which lung tumors were induced by repeated intratracheal instillations of a multiwalled carbon nanotube, MWNT-7. The RNA-seq-based transcriptome identified a large number of significantly differentially expressed genes at Year 0.5, Year 1, and Year 2. Ingenuity Pathway Analysis revealed that macrophage-elicited signaling pathways such as phagocytosis, acute phase response, and Toll-like receptor signaling were activated throughout the experimental period. At Year 2, cancer-related pathways including ERBB signaling and some axonal guidance signaling pathways such as EphB4 signaling were perturbed. qRT-PCR and immunohistochemistry indicated that several key molecules such as Osteopontin/Spp1, Hmox1, Mmp12, and ERBB2 were markedly altered and/or localized in the preneoplastic lesions, suggesting their participation in the induction of lung cancer. Our findings support a scenario of inflammation-induced carcinogenesis and contribute to a better understanding of the molecular mechanism of MWCNT carcinogenicity.

2023-07-17 | GSE234151 | GEO

Lung response to multi-walled carbon nanotube exposure in rats.

Project description:The projected increase in the production and use of nanomaterials is expected to result in a corresponding increase in human exposure, potentially resulting in significant morbidity and mortality. Currently, the lung toxicity of multi-walled carbon nanotubes (MWCNT), a prototype nanomaterial, was investigated in a rat model. The rats were exposed by whole-body inhalation to air (controls) or MWCNT (6 hours/day, 3 days) to result in cumulative doses of 180, 90, 45, 22.5, or 11.25 mg/m3. Lung toxicity and gene expression profiles were determined in the lungs of the control and MWCNT exposed rats one day following termination of the exposures. Moderate lung histological changes, indicative of toxicity, were detected in rats exposed to MWCNT at doses > 45 mg/m3. Changes in toxicity parameters including, lactate dehydrogenase (LDH) activity, oxidant production, cell counts of phagocytes, and cytokine levels were detected in the bronchoalveolar lavage of rats exposed to > 22.5 mg/m3 MWCNT, compared with the air controls. Lung gene expression profiling detected differences in transcript levels (fold change >1.5 and FDR p<0.05) of several genes in the rats exposed to >22.5 mg/m3 MWCNT, compared with the controls. The changes in lung toxicity and gene expression profiles exhibited a dose-response to the MWCNT administered in the rats.

2022-12-31 | GSE148869 | GEO

Lungs of mice exposed to subtoxic doses of nano-titanium dioxide particles by inhalation

Project description:Acute phase reactants serum amyloid A-1, 3 and micro RNA-135b, -449a, and -1 are induced in lungs of mice exposed to subtoxic doses of nano-titanium dioxide particles by inhalation In the present study we investigate pulmonary mRNA and miRNA profiles of mice exposed to subtoxic dose of nano-titanium dioxide particles by inhalation. We show dramatic induction of acute phase reactants, chemoattractants, immune and host defence related genes. We also demonstrate for the first time changes in miRNA profiles in the lungs in response to nanoTiO2. Keywords: Toxicology, disease state analysis, biomarkers of health effects Female C57BL/6 mice were exposed to 40 mg nanoTiO2/m3 for one hour/day for 11 consecutive days and were sacrificed 5 days following the last exposure. Left lung lobes and liver were removed and flash frozen. Total RNA was isolated from a small random part of the frozen lung and liver and was hybridized against universal mouse reference RNA to Agilent Oligo DNA microarrays (Agilent Technologies) containing 44,000 transcripts. Microarrays were normalized using a global LOWESS approach and analyzed by MAANOVA 2.0 and SAM. Microarray results were validated by real time RT-PCR. Impact of alteration in expression of select genes was further validated by analysing their total protein levels in lung tissue homogenates.

2010-12-13 | E-GEOD-19487 | biostudies-arrayexpress

Inhalation of rod-like carbon nanotubes causes unconventional asthma

Project description:Exposure to rod-shaped carbon nanotubes reveals novel innate immunity mediated asthma-like inflammation in mice. Inhalation of rCNT elicited a drastic infiltration of eosinophils into the lungs but only minor infiltration of neutrophils and lymphocytes. Transcriptomic study revealed remarkable effects of rCNT exposure, affecting almost exclusively pathways essential for innate immunity.

2014-10-01 | GSE50176 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data