Ontology highlight
ABSTRACT:
PROVIDER: PRJNA672229 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12904530_1.fastq.gz | Fastqsanger.gz | |||
| SRR12904530_2.fastq.gz | Fastqsanger.gz | |||
| SRR12904531_1.fastq.gz | Fastqsanger.gz | |||
| SRR12904531_2.fastq.gz | Fastqsanger.gz | |||
| SRR12904532_1.fastq.gz | Fastqsanger.gz |
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Project description:Entamoeba histolytica trophozoites epigenetically silenced for ameobapore A (G3 parasites) were additionally silenced for the Rhomboid gene Rom1 (EHI_197460) using the mechanism outlined in Mirelman et al. Parasite. 2008 Sep;15(3):266-74 (PMID 18814693). Rom1 silencing was confirmed by RT-PCR. Gene expression in these parasites was compared to that of the parent G3 strain.
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Project description:Entamoeba histolytica trophozoites epigenetically silenced for ameobapore A (G3 parasites) were additionally silenced for the Rhomboid gene Rom1 (EHI_197460) using the mechanism outlined in Mirelman et al. Parasite. 2008 Sep;15(3):266-74 (PMID 18814693). Rom1 silencing was confirmed by RT-PCR. Gene expression in these parasites was compared to that of the parent G3 strain. RNA was isolated from axenically grown G3 and Rom1 silenced parasites, and hybridized to a custom Affymetrix array for E. histolytica. Two biological replicates were performed for each condition.
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| PRJDB14292 | ENA