Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq Analysis of Wild Type and Mark3-/- mouse primary osteoblasts

ABSTRACT: RNA-seq Analysis of Wild Type and Mark3-/- mouse primary osteoblasts

PROVIDER: PRJNA673179 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12935231_1.fastq.gz	Fastqsanger.gz
	SRR12935231_2.fastq.gz	Fastqsanger.gz
	SRR12935232_1.fastq.gz	Fastqsanger.gz
	SRR12935232_2.fastq.gz	Fastqsanger.gz
	SRR12935233_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

RNA-seq Analysis of Wild Type and Mark3-/- mouse primary osteoblasts

Project description:Bone mineral density (BMD) is a highly heritable predictor of osteoporotic fracture. Genome wide association studies (GWAS) have identified hundreds of loci influencing BMD, but few have been functionally analyzed. In this study, we show that SNPs within a BMD locus on Chromosome 14q32.32 alter splicing and expression of PAR-1a/MARK3, a conserved serine/threonine kinase known to regulate bioenergetics, cell division and polarity. Mice lacking Mark3 either globally or selectively in osteoblasts have increased bone mass at maturity. RNA profiling from Mark3 deficient osteoblasts suggested changes in the expression of components of the Notch signaling pathway. Mark3 deficient osteoblasts exhibited greater matrix mineralization compared with controls that was accompanied by reduced Jag1/Hes1 expression and diminished downstream JNK signaling. Overexpression of Jag1 in Mark3 deficient osteoblasts both in vitro and in vivo normalized mineralization capacity and bone mass, respectively. Together, these findings reveal a mechanism whereby genetically regulated alterations in Mark3 expression perturb cell signaling in osteoblasts to influence bone mass.

2020-10-30 | GSE160415 | GEO

mRNA expression in primary osteoblasts from wild-type and Fam102a knockout mice

Project description:We examined a global mRNA expression in primary osteoblasts from wild-type and Fam102a knockout mice.

2024-12-03 | GSE211533 | GEO

mRNA expression in primary osteoblasts from wild-type and Fam102a knockout mice

Project description:mRNA expression in primary osteoblasts from wild-type and Fam102a knockout mice

| PRJNA870751 | ENA

Expression data from primary osteoblasts isolated from calvaria of Gnptab(c.3082insC) knock-in mice compared with osteoblasts isolated from wild-type littermates

2014-01-29 | GSE43854 | GEO

Expression data from primary osteoblasts isolated from calvaria of Gnptab(c.3082insC) knock-in mice compared with osteoblasts isolated from wild-type littermates

Project description:Mucolipidosis type II (MLII) is a severe inherited multisystemic disorder caused by mutations in the GNPTAB gene. Skeletal abnormalities are a predominant feature of MLII. Here we investigate the gene expression in a knock-in mouse model for mucolipidosis type II, generated by the insertion of a cytosine in the murine Gnptab gene (c.3082insC) that is homologous to a homozygous mutation in an MLII patient. Since osteoblasts are critically involved in regulating bone development and remodeling, a genome-wide expression analysis was performed with RNA isolated from primary cultures of osteoblasts originating from MLII knock-in mice (KI) compared to RNA from wild-type (WT) osteoblasts to identify dysregulated genes involved in pathogenic mechanisms. Primary osteoblasts were isolated from calvaria of 5-day-old wild-type (WT) and MLII knock-in littermates (KI). RNA was extracted at day 10 of differentiation induced by ascorbic acid and beta-glycerophosphate and hybridization on Affymetrix microarrays. We used preparations of RNA from two individual primary cultures of osteoblasts for every genotype (WT_OB_I, WT_OB_II, KI_OB_I, KI_OB_II) and compared WT vs KI samples.

2014-01-29 | E-GEOD-43854 | biostudies-arrayexpress

RNA-Seq of primary CKD osteoblasts

Project description:RNA-Seq of primary CKD osteoblasts

| PRJNA937773 | ENA

Differentiation Time Course Analysis of Gene Expression in Primary Osteoblasts from Wild Type and β-Arrestin 2 Knockout Mice

Project description:Differentiation Time Course Analysis of Gene Expression in Primary Osteoblasts from Wild Type and β-Arrestin 2 Knockout Mice

| PRJNA752103 | ENA

Comparision of expression profile between wild-type and Slc39a13 knockout osteoblasts

Project description:In order to explore molecules whose expression is controlled by Slc39a13, we investigated gene expression profiling of primary osteoblast isolated from wild-type and Slc39a13 knockout mice. Keywords: knockout vs wild-type

2008-11-07 | GSE10555 | GEO

Primary Human Osteoblasts miRNA array

Project description:A microRNA array was performed using human primary osteoblasts (hOB) obtained from trabecular bone of postmenopausal women after knee replacement due to osteoarthritis in order to determine the miRNAs expressed in these osteoblastic cells.

2015-12-21 | GSE74211 | GEO

Basal gene expression of primary osteoblasts

Project description:Osteoblasts derived from different functional skeletal sites were analysed for gene expression profiles Keywords: Cell type comparison

2008-11-15 | GSE13607 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data