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Differential gene expression in a mouse model for pediatric high grade glioma encoding for H3.3-G34R mutation

ABSTRACT: Differential gene expression in a mouse model for pediatric high grade glioma encoding for H3.3-G34R mutation

PROVIDER: PRJNA673221 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12938581_1.fastq.gz	Fastqsanger.gz
	SRR12938581_2.fastq.gz	Fastqsanger.gz
	SRR12938582_1.fastq.gz	Fastqsanger.gz
	SRR12938582_2.fastq.gz	Fastqsanger.gz
	SRR12938583_1.fastq.gz	Fastqsanger.gz

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Differential gene expression in a mouse model for pediatric high grade glioma encoding for H3.3-G34R mutation

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Project description:Sequencing of paediatric gliomas has identified two common substitution mutations (K27M and G34R) in genes encoding histone H3.3. We introduced a single-copy H3.3 G34R targeted mutation in mouse ES cells and observed gains in H3K36me3 and H3K9me3 across the genome. Altered chromatin profiles correlated with enrichment of KDM4 A/B/C, a histone lysine (K9/K36) demethylase. RNA-seq of H3.3 G34R mutant showed disrupted gene expression patterns which also correlated with KDM4 enrichment. Expression of a single copy of H3.3 G34R at endogenous levels was sufficient to genocopy KDM4 triple-KO cells as determined by ChIP-seq and RNA-seq.

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