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RNA Sequencing for MKRN3 regulating genes identification in GT1-7 cells

ABSTRACT: RNA Sequencing for MKRN3 regulating genes identification in GT1-7 cells

PROVIDER: PRJNA673245 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12939278_1.fastq.gz	Fastqsanger.gz
	SRR12939278_2.fastq.gz	Fastqsanger.gz
	SRR12939279_1.fastq.gz	Fastqsanger.gz
	SRR12939279_2.fastq.gz	Fastqsanger.gz
	SRR12939280_1.fastq.gz	Fastqsanger.gz

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RNA Sequencing for MKRN3 regulating genes identification in GT1-7 cells

Project description:Purpose: The deletion or loss-of-function of MKRN3 were genetically associated with human centeral precocious puberty (CPP).The goal of this study is to explore the global genes expression changes on MKRN3 overexpessing by high-throughput RNA sequencing analysis. Methods: The mRNA profiles of wild-type (WT) and MKRN3 overexpressed GT1-7 cells were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were aligned to mus musculus genomic reference (GRCm38) using HISAT2. Read counts were summarized at the gene level in each sample with HTSeq. Results: Using an optimized data analysis workflow, we mapped about 19 million sequence reads per sample to the mouse genome (build mm10) and identified 13,041 transcripts in the GT1-7 cells of WT and MKRN3. Approximately 6% of the transcripts showed differential expression between the WT and MKRN3 overexpressed cells, with a fold change ≥2 and p-adjust value <0.05. 354 genes were up-regulated by MKRN3, while 395 genes were down-regulated by MKRN3 in this study. Conclusions: Our study is the first effort to explore MKRN3 on gobal genes expression, and hundreds of different expressed genes have been identified.

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