Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles in human duodenal organoid-derived monolayer

ABSTRACT: Gene expression profiles in human duodenal organoid-derived monolayer

PROVIDER: PRJNA674027 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profiles in human duodenal organoid-derived monolayer

Project description:The human small intestine is the key organ for absorption, metabolism, and excretion of orally administered drugs. To preclinically predict these reactions in drug discovery research, a cell model that can precisely recapitulate the in vivo human intestinal monolayer is desired. Here, we developed a monolayer platform using human biopsy-derived duodenal organoids for application to pharmacokinetic studies. The human duodenal organoid-derived monolayer was prepared by a simple method in 3–8 days. It consisted of polarized absorptive cells and had a barrier function. It showed much higher cytochrome P450 (CYP) 3A4 and carboxylesterase (CES) 2 activities than Caco-2 cells. It also showed efflux activity of P-glycoprotein (P-gp) and inducibility of CYP3A4. Finally, its gene expression profile was closer to the adult human duodenum, compared to the profile of Caco-2 cells. Based on these findings, this monolayer assay system using biopsy-derived human intestinal organoids is likely to be widely adopted.

2021-05-19 | GSE160695 | GEO

Gene expression profiles in human liver organoid-derived hepatocytes

Project description:Gene expression profiles in human liver organoid-derived hepatocytes

| PRJNA981479 | ENA

Bulk RNA-seq of MilliporeSigma 3DGro human patient-derived gastrointestinal organoid lines compared to SCC300 human iPSC-derived colon organoid line

Project description:Transcriptomic profiles of 6 commercially-available human patient-derived gastrointestinal organoid lines were obtained and compared to transcriptomic profile of a commercially available human iPSC-induced colon organoid line. Transcriptomic profile of iPSC-derived human colon organoid line was compared after culture in either Corning growth-factor-reduced Matrigel (Corning 356231) or MilliporeSigma growth-factor-reduced ECMGel (E6909)

2022-03-31 | E-MTAB-11557 | biostudies-arrayexpress

Single cell analysis of Monolayer and Organoid Primary Prostate Human Epithelial Cells Derived from the same patient

Project description:Single cell analysis of Monolayer and Organoid Primary Prostate Human Epithelial Cells Derived from the same patient

| PRJNA535490 | ENA

Multiplexed single cell mRNA sequencing of monolayer- and organoid-system differentiated cells derived from human iPSC with and without NKX2-5 genetic manipulations

Project description:Three wild type cell lines (WTC:ACTN2-eGFP, WTC:Myl2-eGFP, SCVI114) and three genetically modified cell lines (PM28, PM52, Del33) were differentiated using two monolayer (atrial and ventricular) and two organoid (also atrial and ventricular) differentiation protocols. Cells from multiple samples at differentiation day 15 (wild type cell lines) and day 30 (all cell lines) were isolated, multiplexed using the MULTI-seq approach; subsequently library preparation (10X platform) and sequencing was performed.

2022-05-04 | GSE163619 | GEO

Duodenal adenoma/adenocarcinomas vs matched normal mucosa

Project description:Background: Duodenal adenoma/adenocarcinomas are rare, and the global gene expression changes associated with the initial stages of carcinogenesis of these neoplasms have not been elucidated. Results: To comprehensively analyze genetic markers and pathways specific to early-stage duodenal adenoma/adenocarcinomas, transcriptional profiles of 4 fresh-frozen non-ampullary duodenal adenoma/adenocarcinomas and surrounding duodenal normal mucosa were compared. Key features of gene expression analysis demonstrated a strong correlation between these tumors and colorectal adenomas, as well as the Wnt/β-catenin pathway. These results shed new light on the transcriptional changes that occur during the early stages of duodenal tumorigenesis. All samples were obtained prior to treatment in order to minimize effects of cauterization, and immediately fresh-frozen.

2018-08-06 | GSE102208 | GEO

Duodenal transcriptome profiles of pigs divergent in feed efficiency

Project description:Duodenal transcriptome profiles of pigs divergent in feed efficiency

| PRJNA430453 | ENA

Patient-derived breast cancer organoid study

Project description:Patient-derived breast cancer organoid study

| PRJNA786253 | ENA

Patient-derived breast cancer organoid study

Project description:Patient-derived breast cancer organoid study

| PRJNA786254 | ENA

Gene expression profiling of human iPSC kidney organoid-derived proximal tubules

Project description:The kidney organoid differentiation protocol takes induced pluripotent stem cells through to kidney organoid via directed differentiation in approximately 25 days. The cells are grown in a monolayer in a dish for seven days and are subjected to growth factors before being pelleted on day seven. The organoids then continue to differentiate as a 3D structure, with at least 8 distinct kidney cell types identifiable around day 18. Here proximal tubules were isolated from day 25 kidney organoids and RNA-sequencing libraries generated.

2018-10-31 | GSE99582 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data