Project description:Transcriptional profiling of Arabidopsis thaliana cotyledons comparing ecotype Col-0 (Control) with lea13 T-DNA line to elucidate the response mechanism to drought stress conditions that rely on LEA protein function.
Project description:Transcriptional profiling of Arabidopsis buds comparing dyt1, tdf1, ams, ms188 mutants with wild type (Col ecotype), respectively. Goal was to determine the differential expression genes between the buds of mutant and wild type.
Project description:A dye swap experiment to compare Arabidopsis thaliana ecotype Col-0 with Arabidopsis thaliana ecotype Ler, both grown to Boyes scale 1.04 at VIB, Plant systems biology, Gent, Belgium
Project description:Transcriptional profiling of Arabidopsis buds comparing ahl16 mutants with wild type (Col-0 ecotype). Goal was to determine the differential expression genes between the buds of mutant and wild type.
Project description:This study assess the biocompatibility of Ti-26Nb compared to Ti-Cp, a reference in implantology, through the combination of conventional toxicological assay, morphological observations and transcriptomic analysis performed on two different cells line : Saos-2 and THP-1. The biocompatibility of Ti-Cp implants was evaluated in comparison with Ti-26Nb implants both having a mirror polished surface, studying cell proliferation (trypan blue and WST-1) and cell morphology/adhesion (SEM) on human THP-1 and Saos-2 cell lines. Finally, an evaluation of a potential toxicity of potassium niobiate powder KNbO3 was carried out by measuring metabolic activity and realizing a transcriptomic study on the THP-1 line (the first one) to deepen the results observed with Ti-26Nb discs. Indeed, niobiate ions are potentially released from implant in cellular acidic environnement. Concerning Saos-2 cells, our results suggest that Ti-26Nb and Ti-Cp discs do not impact proliferation and viability. Concerning THP-1 cell line, a decrease in proliferation and viability is observed in contact of Ti-26Nb discs (hypothesis of a cell-line effect ?, no biocompatibility study on monocyte/macrophage cell lines such as THP-1 actually available in literature). KNbO3 powder does not impact viability of THP-1 cells and does not induce changes at molecular level. Taken together, these data suggest a possible toxicity of Ti-26Nb toward THP-1 cells not directly related to the niobium but perhaps to the manufacturing process of Titane niobium alloy.
Project description:Treatment of Arabidopsis thaliana (ecotype Col-0) seedlings with jasmonic acid (JA) elicits long-term induced resistance (IR) against the chewing herbivore Spodoptera littoralis. We used whole genome bisulfite-seq to profile the methylome associated with this long-lasting JA-IR.
Project description:Treatment of Arabidopsis thaliana (ecotype Col-0) seedlings with jasmonic acid (JA) elicits long-term induced resistance (IR) against the chewing herbivore Spodoptera littoralis. We used RNA-seq to profile the transcriptional changes associated with this long-lasting JA-IR.
Project description:A dye swap experiment to compare Arabidopsis thaliana ecotype Col-0 grown to stage 1.04 (Boyes scale) at VIB, Plant systems biology, Gent, Belgium with Arabidopsis thaliana ecotype Col-0 grown to stage 1.04 (Boyes scale) at FU Berlin.