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Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [RNA-Seq]

ABSTRACT: Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [RNA-Seq]

PROVIDER: PRJNA674857 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12993463_1.fastq.gz	Fastqsanger.gz
	SRR12993463_2.fastq.gz	Fastqsanger.gz
	SRR12993464_1.fastq.gz	Fastqsanger.gz
	SRR12993464_2.fastq.gz	Fastqsanger.gz
	SRR12993465_1.fastq.gz	Fastqsanger.gz

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Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization

Project description:Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization

| PRJNA674852 | ENA

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [ATAC-seq]

Project description:Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [ATAC-seq]

| PRJNA669853 | ENA

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization.

Project description:In the fruit fly Drosophila melanogaster, male-specific splicing and translation of the Fruitless transcription factor (FruM) alters the presence, anatomy, and/or connectivity of >60 types of central brain neurons that interconnect to generate male-typical behaviors. While the indispensable function of FruM in sex-specific behavior has been understood for decades, the molecular mechanisms underlying its activity remain unknown. Here, we take a genome-wide, brain-wide approach to identifying regulatory elements whose activity depends on the presence of FruM. We identify 436 high-confidence genomic regions differentially accessible in male fruitless neurons, validate candidate regions as bona fide, differentially regulated enhancers, and describe the particular cell types in which these enhancers are active. We find that individual enhancers are not activated universally but are dedicated to specific fru+ cell types. Aside from fru itself, genes are not dedicated to or common across the fru circuit; rather, FruM appears to masculinize each cell type differently, by tweaking expression of the same effector genes used in other circuits. Finally, we find FruM motifs enriched among regulatory elements that are open in the female but closed in the male. Together, these results suggest that FruM acts cell-type-specifically to decommission regulatory elements in male fruitless neurons.

| S-EPMC7924761 | biostudies-literature

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-01-22 | GSE160931 | GEO

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [RNA-Seq]

Project description:Gene expression profile of neurons comprising most of the central brain courtship circuit in adult male Drosophila brains, with sex and cell-type control populations.

2021-01-22 | GSE160930 | GEO

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [ATAC-seq]

Project description:Chromatin accessibility of neurons comprising most of the central brain courtship circuit in adult male Drosophila brains, with sex and cell-type control populations.

2021-01-22 | GSE159600 | GEO

Molecular Characterization of a Partial Masculinization in Embryonic Ovaries Grafted into Male Nude Mice

Project description:The fetal ovarian grafts under the kidney capsule of adult male mice undergo a partial sex-reversal showing ectopic SOX9-positive Sertoli cell-like cells around 15-20 days post-transplantation. However, the molecular bases of such masculinization of fetal ovaries in the paternal environment were unclear. We examined the temporal changes of the gene expression profiles of the grafted fetal ovaries during the partial masculinizing process in the male nude mice.

2019-02-04 | GSE88797 | GEO

Anti-masculinization induced by aromatase inhibitors in adult female zebrafish

Project description:Anti-masculinization induced by aromatase inhibitors in adult female zebrafish

| PRJNA596764 | ENA

Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains

Project description:Drosophila melanogaster adult males perform an elaborate courtship ritual to entice females to mate. fruitless (fru), a gene that is one of the key regulators of male courtship behavior, encodes multiple male-specific isoforms (FruM). These isoforms vary in their carboxy-terminal zinc finger domains, which are predicted to facilitate DNA binding. By over-expressing individual FruM isoforms in fru-expressing neurons in either males or females and assaying the global transcriptional response by RNA-sequencing, we show that three FruM isoforms have different regulatory activities that depend on the sex of the fly. We identified several sets of genes regulated downstream of FruM isoforms.

2013-09-30 | GSE50515 | GEO

Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules

Project description:Drosophila reproductive behaviors are directed by fruitless neurons. A reanalysis of genomic studies shows that genes encoding dpr and DIP Immunoglobulin superfamily (IgSF) members are expressed in fru P1 neurons. We find that each fru P1 and dpr/DIP (fru P1 ∩ dpr/DIP) overlapping expression pattern is similar in both sexes, but there are dimorphisms in neuronal morphology and cell number. Behavioral studies of fru P1 ∩ dpr/DIP perturbation genotypes indicates that the mushroom body functions together with the lateral protocerebral complex to direct courtship behavior. A single-cell RNA-seq analysis of fru P1 neurons shows that many DIPs have high expression in a small set of neurons, whereas the dprs are often expressed in a larger set of neurons at intermediate levels, with a myriad of dpr/DIP expression combinations. Functionally, we find that perturbations of sex hierarchy genes and of DIP-ε change the sex-specific morphologies of fru P1 ∩ DIP-α neurons.

2021-02-23 | GSE162098 | GEO

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