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Clitopilus hobsonii strain:QYL-10

ABSTRACT: Clitopilus hobsonii strain:QYL-10 Genome sequencing

PROVIDER: PRJNA675211 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR13072276_1.fastq.gz	Fastqsanger.gz
	SRR13072276_2.fastq.gz	Fastqsanger.gz
	SRR13072277_subreads.fastq.gz	Fastqsanger.gz
	SRR13089179_1.fastq.gz	Fastqsanger.gz

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Project description:We knocked out the ChATG8 gene of Colletotrichum higginsianum IMI349063 using Agrobacterium-mediated transformation technology and obtained the Chatg8Δ mutant, and subsequently collected mycelium from the WT strain after 4 days of incubation, the WT strain after 10 days of incubation, and the Chatg8Δ mutant after 10 days of incubation, respectively, for proteome sequencing.

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Transcriptome Comparison of phoU Homologies: Genes Deletion strain and the Parent Strain in stationary phase (10 h)

Project description:Purpose: To compare the transcriptome changes in phoU1(serp0956) or phoU2(serp0316)deletion strain with the parent strain SE1457 in stationary phase (10 h). Methods: Total RNA was isolated and sequenced from the phoU1 deletion strain, phoU2 deletion strain and the parent strain SE1457 in stationary phase (10 h) in triplicate using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which performed fold-change>=1.5 and FDR<=0.05. Changed genes were then filtered to reveal the downstream targets of phoU1 or phoU2. Results: The expression of 2 genes were significantly decreased and no genes was significantly increased in expression level due to the loss of phoU1 expression.The expression of 279 genes were significantly decreased and 716 genes were significantly increased in expression level due to the loss of phoU2 expression.

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