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Pseudomonas fluorescens Q8r1-96

ABSTRACT: Pseudomonas fluorescens Q8r1-96 genome sequencing

PROVIDER: PRJNA67537 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	AHPO01.dat.gz	Other
	AHPO01.fasta.gz	Fasta.gz
	AHPO01.master.dat	Other
	AHPO01.dat.gz	Other

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Pseudomonas brassicacearum strain:Q8r1-96

Project description:Pseudomonas brassicacearum Q8r1-96 transcriptome - Q8r1-96REb

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Pseudomonas brassicacearum strain:Q8r1-96

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Project description:This SuperSeries is composed of the following subset Series: GSE29319: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(II) chloride GSE29320: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(III) chloride Refer to individual Series

2011-05-16 | E-GEOD-29322 | biostudies-arrayexpress

Structural and functional analysis of the type III secretion system from Pseudomonas fluorescens Q8r1-96.

Project description:Pseudomonas fluorescens Q8r1-96 represents a group of rhizosphere strains responsible for the suppressiveness of agricultural soils to take-all disease of wheat. It produces the antibiotic 2,4-diacetylphloroglucinol and aggressively colonizes the roots of cereal crops. In this study, we analyzed the genome of Q8r1-96 and identified a type III protein secretion system (T3SS) gene cluster that has overall organization similar to that of the T3SS gene cluster of the plant pathogen Pseudomonas syringae. We also screened a collection of 30 closely related P. fluorescens strains and detected the T3SS genes in all but one of them. The Q8r1-96 genome contained ropAA and ropM type III effector genes, which are orthologs of the P. syringae effector genes hopAA1-1 and hopM1, as well as a novel type III effector gene designated ropB. These type III effector genes encoded proteins that were secreted in culture and injected into plant cells by both P. syringae and Q8r1-96 T3SSs. The Q8r1-96 T3SS was expressed in the rhizosphere, but mutants lacking a functional T3SS were not altered in their rhizosphere competence. The Q8r1-96 type III effectors RopAA, RopB, and RopM were capable of suppressing the hypersensitive response and production of reactive oxygen species, two plant immune responses.

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Expression analysis of Pseudomonas fluorescens Pf0-1 (Wt) and delta-pst mutant grown in K10T-1 medium

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2011-11-24 | E-GEOD-33909 | biostudies-arrayexpress

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