Project description:The Pseudomonas synxantha strain NCIMB10586 produces the antibiotic mupirocin / Pseudomonic acid A from a 75 kb gene cluster; expression of this is regulated through quorum-sensing. We wished to examine expression of the strain during batch growth, in particular of the mupirocin cluster and any other operons regulated in a similar manner. We took samples from three independent cultures at 7 time-points - sampling every 2 hours from 6 to 18 hours.