Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CAMP and mGluR1/5 dependent expression of mRNA, lncRNA, and miRNA in mouse hippocampal neurons

ABSTRACT: cAMP and mGluR1/5 dependent expression of mRNA, lncRNA, and miRNA in mouse hippocampal neurons

PROVIDER: PRJNA675483 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13010000_1.fastq.gz	Fastqsanger.gz
	SRR13010000_2.fastq.gz	Fastqsanger.gz
	SRR13010001_1.fastq.gz	Fastqsanger.gz
	SRR13010001_2.fastq.gz	Fastqsanger.gz
	SRR13010002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 57

Similar Datasets

cAMP and mGluR1/5 dependent expression of mRNA, lncRNA, and miRNA in mouse hippocampal neurons

Project description:To identify cAMP-dependent and mGluR1/5-dependent RNAs, we treated DIV16 primary hippocampal neurons with 50uM Forskolin, 30uM DHPG, or equal volume DMSO for 30 minutes prior to RNA isolation using Trizol and Total/small RNA sequencing. We submitted samples to Scripps Florida Genomics Core for library preparation and sequencing.

2022-11-07 | GSE161071 | GEO

cAMP dependent gene expression in mouse hippocampal neurons

Project description:cAMP dependent gene expression in mouse hippocampal neurons

| PRJNA661096 | ENA

cAMP dependent gene expression in mouse hippocampal neurons

Project description:To identify cAMP-dependent lncRNAs, we treated DIV16 primary hippocampal neurons with 50uM Forskolin or equal volume DMSO for 30 minutes prior to RNA isolation using Trizol and Total RNA sequencing. We submitted samples to Scripps Florida Genomics Core for library preparation and sequencing.

2021-02-26 | GSE157388 | GEO

Microarray screening of transcription factors in hippocampal neurons transduced with AAV2-GFP-CBD

Project description:We have analyzed how interaction of calcineurin with perinuclear signalosome AKAP6 affects the expression profile of transcription factors involved in cAMP/calcium signaling. We have found that calcineurin interacts with AKAP6 in calcium-dependent manner and by using anchoring disruptors, we demonstrated the importance of this interaction for depolarization-induced outgrowth of hippocampal neurons.

2024-12-12 | E-MTAB-14613 | biostudies-arrayexpress

Identification of the miRNA targetome in hippocampal neurons using RIP-seq

Project description:We established a neuron-specific Argonaute2:GFP-RNA immunoprecipitation followed by high throughput sequencing (AGO2-RIP-seq) to analyse the regulatory role of miRNAs in mouse hippocampal neurons. Using this technique, we identified more than two thousand miRNA target genes in hippocampal neurons, regulating essential neuronal features such as axon guidance and transcription. Furthermore, we found that stable inhibition of the highly expressed miR-124 in hippocampal neurons led to significant changes in the AGO2 binding of target mRNAs, resulting in subsequent upregulation of numerous miRNA target genes. Our data suggest that target redundancies are common among microRNA families. Together, these findings greatly enhance our understanding of the mechanisms and dynamics through which miRNAs regulate their target genes in neurons. Analysis of the miRNA targetome in hippocampal neurons after inhibition of 2 different miRNAs. AAV5 injections into the hippocampus of adult C57BL/6 mice producing either of the following under a synapsin promoter: GFP only (Samples beginning with 'GFP124…' or 'GFP125…'), GFP-miR124sp (Samples beginning with 'miR124…'), GFP-miR125sp (Samples beginning with 'miR125…'), GFP-AGO2-miR292sponge (samples ending with '…292'), GFP-AGO2-miR124sponge (samples ending with '…124'), GFP-AGO2-miR125sponge (samples ending with '…125'). All other samples were sham-injected.

2015-07-31 | E-GEOD-65543 | biostudies-arrayexpress

Identification of the miRNA targetome in hippocampal neurons using RIP-seq

2015-07-31 | GSE65543 | GEO

Rat hippocampal neurons' proteomes

Project description:We induced over-expression and under-expression of Camk2b in cultured rat hippocampal neurons through transfection with lentivirus plasmids. Then isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics followed by bioinformatics analyses were carried out to explore the impacts of Camk2b dysexpression on the proteome of the neurons.

2022-07-27 | PXD033931 |

Primary Cilia-Autophagy Axis in Hippocampal Neurons Is Essential to Maintain Cognitive Fitness

Project description:A central hallmark of brain aging is the alteration of neuronal functions in the hippocampus, leading to a progressive decline in learning and memory. Multiple reports have shown the importance of blood-borne factors in inter-tissue communication for the maintenance of cognitive fitness and proper regulation of neuronal homeostasis throughout life. Among these blood-borne factors, we identified Osteocalcin (OCN), a bone-derived hormone. OCN induces autophagy machinery in hippocampal neurons which is essential for activity-dependent synaptic plasticity. However, the way in which blood-borne factors like OCN communicate with neurons, including their regulatory mechanisms, remains largely elusive. Here, we show the importance of a core primary cilium (PC)-proteins/autophagy machinery axis in hippocampal neurons that mediate the effects of the pro-youthful blood factor OCN on neuronal homeostasis and cognitive fitness. We found that OCN’s receptor, GPR158, is present at the PC of hippocampal neurons and mediates the regulation of autophagy machinery by OCN. During aging, PC-core proteins are reduced in hippocampal neurons and associated with neuronal PC morphological abnormalities. Restoring their levels is sufficient to improve neuronal autophagy and cognitive impairments in aged mice. Mechanistically, we found that OCN promotes neuronal autophagy in the hippocampus by the induction of PC-dependent cAMP response element-binding protein (CREB) signaling pathway. Altogether, this study proposes a novel paradigm for blood factor-neuron communication dependent on a neuronal PC/autophagy axis by identifying a novel regulatory pathway fostering cognitive fitness and providing the foundation for autophagy-based therapeutic strategies to treat age-related cognitive dysfunction.

2024-11-07 | PXD056975 | Pride

Contextual fear conditioning induced transcriptional changes in hippocampal CA1 neurons

Project description:The goal of our study was to assess whether the experience can regulate specific lncRNAs within the hippocampus and their role in associative memory. To address this, we carried out unbiased analyses of gene expression in CA1-hippocampal neurons to identify lncRNA changes induced by contextual fear conditioning (CFC).

2023-08-07 | GSE214838 | GEO

miRNA profiling array and analysis in mouse hippocampal neurons

Project description:We addressed the potential for global regulation of miRNA biogenesis by BDNF using miRNA arrays that selectively measure mature miRNA, as opposed to pre-miRNA. Hippocampal neurons were treated with BDNF for 30 min in the presence of Actinomycin-D to assess changes due to processing of existing pre-miRNAs rather than new pre-miRNA production. We used Applied Biosystems 7900HT Fast Real-Time PCR system using Taqman Rodent MicroRNA Array A. Data is from three paired BDNF and Mock experiments (1,2,3). Each array (TaqMan) contained 375 rodent miRNA targets of which 195 were detectable in hippocampus in three independent paired experiments.

2012-03-02 | E-GEOD-35969 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data