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ABSTRACT: A marine bacterial community that degrades poly (ethylene terephthalate) and polyethylene

PROVIDER: PRJNA675535 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13011673_1.fastq.gz	Fastqsanger.gz
	SRR13011673_2.fastq.gz	Fastqsanger.gz
	SRR13011674_1.fastq.gz	Fastqsanger.gz
	SRR13011674_2.fastq.gz	Fastqsanger.gz
	SRR13011675_1.fastq.gz	Fastqsanger.gz

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Multiomics study to elucidate the biodegradation of copolyester film by a marine microbial enrichment culture

Project description:Although the biodegradation of biodegradable plastics in soil and compost is well-studied, there is little knowledge on the metabolic mechanisms of synthetic polymers degradation by marine microorganisms. Here, we present a multiomics study to elucidate the biodegradation mechanism of a commercial aromatic-aliphatic copolyester film by a marine microbial enrichment culture. The plastic film and each monomer can be used as sole carbon source. Our analysis showed that the consortium synergistically degrades the polymer, different degradation steps being performed by different members of the community. Analysis of gene expression and translation profiles revealed that the relevant degradation processes in the marine consortium are closely related to poly(ethylene terephthalate) biodegradation from terrestrial microbes. Although there are multiple genes and organisms with the potential to perform a degradation step, only a few of these are active during biodegradation. Our results elucidate the potential of marine microorganisms to mineralize biodegradable plastic polymers and describe the mechanisms of labor division within the community to get maximum energetic yield from a complex synthetic substrate.

2020-09-25 | PXD018391 | Pride

Project description:A marine bacterial community that degrades polyethylene

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Project description:Synthetic plastics, like polyethylene terephthalate (PET), have become an essential part of modern life. Many of these products are remarkably persistent in the environment, and the accumulation in the environment is recognised as a major threat. Therefore, an increasing interest has been paid to screen for organisms able to degrade and assimilate the plastic. Ideonella sakaiensis was isolated from a plastisphere, a bacterium that solely was thriving on the degradation on PET films. The processes affected by the presence of PET, terephthalic acid, ethylene glycol, ethyl glycolate, and sodium glyoxylate monohydrate was elucidated by differential proteomes. The exposure of PET and its monomers seem to affect two major pathways, the TCA cycle and the β-oxidation pathway, since multiple of the conditions resulted in an increased expression of proteins directly or indirectly involved in these pathways, underlying the importance in the degradation of PET by I. sakaiensis.

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Polyethylene terephthalate and its monomers degradation by Ideonella sakaiensis (secreted)

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RNA-seq of Ideonella sakaiensis 201-F6 when grown on PET, TPA·2Na, and EG

Project description:Poly(ethylene terephthalate) (PET)-degrading bacterium Ideonella sakaiensis produces hydrolytic enzymes that convert PET, via mono(2-hydroxyethyl) terephthalate (MHET), into the monomeric compounds, terephthalic acid (TPA) and ethylene glycol (EG). Understanding PET metabolism is critical if this bacterium is to be engineered for bioremediation and biorecycling. TPA uptake and catabolism in I. sakaiensis have previously been studied, but EG metabolism remains largely unexplored despite its importance. First, we identified two alcohol dehydrogenases (IsPedE and IsPedH) and one aldehyde dehydrogenase (IsPedI) in I. sakaiensis as the homologs of EG metabolic enzymes in Pseudomonas putida KT2440. IsPedE and IsPedH exhibited EG dehydrogenase activities with Ca2+ and a rare earth element (REE) Pr3+, respectively. We further found an upregulated dehydrogenase gene when the bacterium was grown on EG, whose gene product (IsXoxF) displays a minor EG dehydrogenase activity with Pr3+. IsPedE displayed a similar level of activity toward various alcohols. In contrast, IsPedH was more active toward small alcohols, whereas IsXoxF was the opposite. Structural analysis with homology models revealed that IsXoxF had a larger catalytic pocket than IsPedE and IsPedH, which could accommodate relatively bulkier substrates. Pr3+ regulated the protein expression of IsPedE negatively; IsPedH and IsXoxF were positively regulated. Taken together, these results indicated that the combination of IsPedH and IsXoxF complements the function of IsPedE in the presence of REEs. IsPedI exhibited dehydrogenase activity toward various aldehydes with the highest activity toward glycolaldehyde (GAD). This study demonstrated a unique alcohol oxidation pathway of I. sakaiensis, which could be efficient in EG utilization.

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