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Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats

ABSTRACT: Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats

PROVIDER: PRJNA675548 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13010500.fastq.gz	Fastqsanger.gz
	SRR13010501.fastq.gz	Fastqsanger.gz
	SRR13010502.fastq.gz	Fastqsanger.gz
	SRR13010503.fastq.gz	Fastqsanger.gz
	SRR13010504.fastq.gz	Fastqsanger.gz

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Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats [PKD_Jan19]

Project description:Cystic kidney disease (CKD) is a heterogeneous group of genetic disorders and one of the most common causes of end-stage renal disease. Here, we investigate the potential effects of long-term human stem cells treatment on kidney function and the gene expression profile of PKD/Mhm (Cy/+) rats. Human adipose derived stromal cells (ASC) and human skin-derived ABCB5+ stromal cells (2x106) were monthly, over a period of 6 months, infused intravenously or injected intraperitoneally. Additionally, ASC and ABCB5+ derived conditioned media were administrated intraperitoneally. Gene expression profile results showed a significant reprogramming of metabolism related pathways along with the down-regulation of cAMP, NF-B and apoptosis pathways. During the experimental period, the principal renal parameters as well as renal function using an innovative non-invasive transcutaneous device were measured. All together, these analyses show a moderate amelioration of the renal function in ABCB5+ and ASC treated groups. Additionally, ABCB5+ and ASC derived conditioned media treatments lead to milder, but still promising improvements. Cell-based therapy may constitute a novel therapeutic approach in CKD.

2022-05-18 | GSE161092 | GEO

Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats [PKD_Dez19]

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Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats [PKD_Dez19]

Project description:Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats [PKD_Dez19]

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Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats [PKD_Jan19]

Project description:Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats [PKD_Jan19]

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Potential Therapeutic Effects of Long-Term Stem Cell Administration: Impact on the Gene Profile and Kidney Function of PKD/Mhm (Cy/+) Rats.

Project description:Cystic kidney disease (CKD) is a heterogeneous group of genetic disorders and one of the most common causes of end-stage renal disease. Here, we investigate the potential effects of long-term human stem cell treatment on kidney function and the gene expression profile of PKD/Mhm (Cy/+) rats. Human adipose-derived stromal cells (ASC) and human skin-derived ABCB5+ stromal cells (2 × 106) were infused intravenously or intraperitoneally monthly, over 6 months. Additionally, ASC and ABCB5+-derived conditioned media were administrated intraperitoneally. The gene expression profile results showed a significant reprogramming of metabolism-related pathways along with downregulation of the cAMP, NF-kB and apoptosis pathways. During the experimental period, we measured the principal renal parameters as well as renal function using an innovative non-invasive transcutaneous device. All together, these analyses show a moderate amelioration of renal function in the ABCB5+ and ASC-treated groups. Additionally, ABCB5+ and ASC-derived conditioned media treatments lead to milder but still promising improvements. Even though further analyses have to be performed, the preliminary results obtained in this study can lay the foundations for a novel therapeutic approach with the application of cell-based therapy in CKD.

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Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-18 | GSE161093 | GEO

Global gene expression profiling in early-stage polycystic kidney disease in the Han:SPRD Cy rat identifies a role for RXR signaling

Project description:Han:SPRD Cy is a spontaneous rat model of polycystic kidney disease (PKD) caused by a missense mutation in Pkdr1. Cystogenesis in this model is not clearly understood. In the current study, we performed global gene expression profiling in early-stage PKD cyst development in Cy/Cy kidneys and normal (+/+) kidneys, at 3 and 7 days of postnatal age. Expression profiles were determined by microarray analysis, followed by validation with real-time RT-PCR. Genes were selected with over 1.5 fold expression changes compared with age-matched +/+ kidneys for canonical pathway analysis. We found 9 pathways in common between 3-day and 7-day Cy/Cy kidneys. Three significantly changed pathways were designated 'VDR/RXR Activation,' 'LPS/IL-1 Mediated Inhibition of RXR Function,' and 'LXR/RXR Activation'. These results suggest that RXR mediated signaling is significantly altered in developing kidneys with mutated Pkdr1. In gene ontology analysis, the functions of these RXR-related genes were found to be involved in regulating cell proliferation and organ morphogenesis. With real-time RT-PCR analysis, the up-regulation of Ptx2, Alox15b, OSP and PCNA, major markers of cell proliferation associated with the RXR pathway, were confirmed in 3- and 7-day Cy/Cy kidneys compared with 3-day +/+ kidneys. The increased RXR protein was observed both in nuclei and cytoplasm of cystic epithelial cells in early-stage Cy/Cy kidneys, and the RXR-positive cells were strongly positive for PCNA staining. Taken together, cell proliferation and organ morphogenesis signals transduced by RXR mediated pathways may have important roles for cystogenesis in early-stage PKD in this Pkdr1-mutated Cy rat.

2011-12-09 | GSE23079 | GEO

Expression data from kidney of 36d old PKD/Mhm rats.

Project description:Gender has strong impact on kidney performance. Female gender tends to be renal protective. PKD/Mhm is rat model for human polycystic kidney disease (PKD) caused by a mutation in gene Anks6. PKD progresses faster in male rats compared with females. We used microarrays to detect gender-dependent gene expression in kidney of 36d old PKD/Mhm rats. Keywords: gender, genotype

2009-09-30 | GSE13065 | GEO

Expression data from the kidney tissue of Han:SPRD rats

Project description:Han:SPRD rats carry a missense mutation in Anks6 (also called Pkdr1), leading to an R823W substitution in SamCystin, a protein that contains ankyrin repeats and a sterile alpha motif (SAM). Han:SPRD Cy/+ rats develop a slowly progressing form of PKD which resembles phenotypically human ADPKD. We used microarrays to examine alterations in the renal gene expression in a rodent model of PKD, namely the Han:SPRD rat.

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Streptococcus equi subsp. zooepidemicus CY strain:CY

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