Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: NGS of gene using spCas9-SpG variants in rice

PROVIDER: PRJNA675713 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13013760_1.fastq.gz	Fastqsanger.gz
	SRR13013760_2.fastq.gz	Fastqsanger.gz
	SRR13013761_1.fastq.gz	Fastqsanger.gz
	SRR13013761_2.fastq.gz	Fastqsanger.gz
	SRR13013762_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 64

Similar Datasets

Project description:NGS of gene using spCas9 variants (spCas9-NG) in rice

| PRJNA679144 | ENA

Project description:NGS of gene using spCas9 variants in rice-5

| PRJNA679420 | ENA

Project description:NGS of gene using spCas9 variants in rice-3

| PRJNA678612 | ENA

Project description:NGS of gene using spCas9 variants in rice-1

| PRJNA674028 | ENA

Project description:NGS of gene using spCas9 variants in rice-2

| PRJNA676008 | ENA

Project description:NGS of gene using spCas9 variants in rice-4

| PRJNA679425 | ENA

Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2

Project description:Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here, we benchmark PAM preferences, on-target activity, and off-target susceptibility of 11 variants of SpCas9 in cell culture assays with thousands of guides targeting endogenous genes. To enhance the coverage and thus utility of base editing screens, we demonstrate that the SpCas9-NG and SpG variants are compatible with both A>G and C>T base editors, more than tripling the number of guides and assayable residues. We demonstrate the performance of these technologies by screening for loss-of-function mutations in BRCA1 and Venetoclax-resistant mutations of BCL2, identifying both known and new insights into these clinically-relevant genes. We anticipate that the tools and methodologies described here will facilitate the investigation of genetic variants at a finer and deeper resolution for any locus of interest.

2021-07-21 | GSE180351 | GEO

Project description:NGS of gene using SpRY variants in rice-2

| PRJNA674974 | ENA

Project description:NGS of gene using SpRY variants in rice-1

| PRJNA674916 | ENA

Project description:NGS of gene using FrCas9 in rice

| PRJNA1025872 | ENA