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Escherichia coli O104:H4 str. H112180280

ABSTRACT: Escherichia coli O104:H4 str. H112180280 Genome sequencing and assembly

PROVIDER: PRJNA67929 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	AFPN02.dat.gz	Other
	AFPN02.fasta.gz	Fasta.gz
	AFPN02.master.dat	Other
	SRR388806.fastq.gz	Fastqsanger.gz
	SRR388807.fastq.gz	Fastqsanger.gz

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Project description:We performed a high-throughput mapping of the 5’ end transcriptome of the pAA plasmid of the clinical Escherichia coli O104:H4 (E. coli O104:H4) isolate LB226692. We employed differential RNA-sequencing (dRNA-seq), a terminator exonuclease (TEX)-based RNA-seq approach allowing for the discrimination of primary and processed transcripts. This method has proven to be a powerful tool for the mapping of transcription start sites (TSS) and detection of non-coding RNAs (ncRNAs) in bacteria. We catalogued pAA-associated TSS and processing sites on a plasmid-wide scale and performed a detailed analysis of the primary transcriptome focusing on pAA virulence gene expression.

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