Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.
Project description:We identified a novel transcription factor, MsoA, in response to oxidative stress in Mycolicibacterium smegmatis. We hope to discover the regulatory network and target genes of transcription factor msoA by transcriptome sequencing of wild-type and msoA mutants, so as to help us further elucidate the molecular mechanism of msoA involved in regulation of Mycolicibacterium oxidation.
Project description:Metastasis accounts for the leading cause of treatment failure in nasopharyngeal carcinoma (NPC). However, the exact mechanisms underlying metastasis remain elusive. S18 and S26 are a pair of NPC cell lines with high and low metastasis abilities, respectively. To evaluate the expression profiles of mRNA in S18 and S26 cells will help to demonstrate genes that contribute the metastasis of NPC. We performed microarray sequencing to clarify the genes that differentially expressed in S18 and S26 cells.