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DNA replication timing directly regulates the frequency of oncogenic chromosomal translocations [HiC]

ABSTRACT: DNA replication timing directly regulates the frequency of oncogenic chromosomal translocations [HiC]

PROVIDER: PRJNA679569 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13086144_1.fastq.gz	Fastqsanger.gz
	SRR13086144_2.fastq.gz	Fastqsanger.gz
	SRR13086145_1.fastq.gz	Fastqsanger.gz
	SRR13086145_2.fastq.gz	Fastqsanger.gz
	SRR13086146_1.fastq.gz	Fastqsanger.gz

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DNA replication timing directly regulates the frequency of oncogenic chromosomal translocations [HiC]

Project description:Many cancers originate from misregulation of gene expression caused by chromosomal translocations which result from ligation of DNA double-strand breaks (DSBs). Indeed, translocations may be causal in ~20% of human cancer morbidity 1. Although the sources of DSBs are numerous2-5, we have virtually no knowledge of the steps linking DSB formation to DSB ligation. Here, we show that early replication timing of translocation partner loci, mediated by the activity of replication origins, is a critical regulator of lymphomagenic Myc translocations generated by activation-induced deaminase (AID) during antibody maturation in B-cells. Reduced levels of the replicative helicase, the minichromosome maintenance (MCM) complex6, impairs translocation genesis, decreases firing of replication origins at AID target genes and globally abrogates the replication timing program without altering cell proliferation, gene expression or genome architecture. Strikingly, deleting a single origin of replication at Myc induces a switch from early-to-late replication at Myc with concomitantly impaired translocation frequency. This phenotype is reversed by restoring early replication at Myc thereby demonstrating a direct, causal role of replication origin activity and replication timing in translocation genesis. Finally, this replication timing-mediated step acts downstream of DSBs and is independent of DSB frequency, constituting a novel regulatory step in translocation biogenesis.

2022-09-15 | GSE161817 | GEO

DNA replication timing directly regulates the frequency of oncogenic chromosomal translocations

Project description:Chromosomal translocations result from the joining of DNA double-strand breaks (DSBs) and frequentlycause cancer. However, the steps linking DSB formation to DSB ligation remain undeciphered. Wereport that DNA replication timing (RT) directly regulates lymphomagenicMyctranslocations duringantibody maturation in B cells downstream of DSBs and independently of DSB frequency. Depletion ofminichromosome maintenance complexes alters replication origin activity, decreases translocations,and deregulates global RT. Ablating a single origin atMyccauses an early-to-late RT switch, loss oftranslocations, and reduced proximity with the immunoglobulin heavy chain (Igh) gene, its majortranslocation partner. These phenotypes were reversed by restoring early RT. Disruption of early RT alsoreduced tumorigenic translocations in human leukemic cells. Thus, RT constitutes a general mechanismin translocation biogenesis linking DSB formation to DSB ligation.

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