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Streptomyces clavuligerus strain:F1D25

ABSTRACT: Streptomyces clavuligerus strain:F1D25 Genome sequencing

PROVIDER: PRJNA679971 | ENA |

REPOSITORIES: ENA

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Expression data from Streptomyces clavuligerus

Project description:To increase production of the important pharmaceutical compounds, both mutagenesis approaches and rational engineering have been extensively applied. Mutagenesis approaches are most popular in industry, but their effects have not yet been studied very well. Here, we used microarrays to compare the transcriptomes of the S. clavuligerus wild type (ATCC 27064) strain and the DS48802 clavulanic acid high-producer strain, which has been obtained by classical strain improvement (mutagenesis). Streptomyces clavuligerus strains were grown in shake flasks. RNA was extracted after 70h and hybridized to microarrays.

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Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and a mutant altered in antibiotic production

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Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and a mutant altered in antibiotic production

Project description:The objective was to analyze the differential expression between the wild strain and the Streptomyces clavuligerus ΔclaR::aac mutant Six experimental conditions were assayed, two strains (Streptomyces clavuligerus ATCC 27064, S. clavuligerus ΔclaR::aac) in three culture times (22.5h, 46.5h and 60 h). Two biological replicates for each condition.

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Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and mutants altered in the antibiotics production

Project description:The objective is to analyze the differential expression between the wild strain and a ccaR-deleted and oppA2::aph mutants 6 biological conditions were used, three strains in two times (exponential and stationary growth phase; Streptomyces clavuligerus ATCC 27064, S. clavuligerus M-bM-^HM-^FccaR and S. clavuligerus oppA2::aph). Four biological replicates were made for each condition

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Streptomyces clavuligerus Transcriptome using adpA deleted and overexpressed mutants

Project description:RNA seq analysis was performed using adpA-deleted and adpA-overexpressed Streptomyces clavuligerus strains to determine its regulatory effect on tunicamycin and other secondary metabolites production

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Streptomyces clavuligerus strain:F1D18

Project description:Streptomyces clavuligerus strain:F1D18 Genome sequencing

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Streptomyces clavuligerus strain:F1D28

Project description:Streptomyces clavuligerus strain:F1D28 Genome sequencing

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Streptomyces clavuligerus strain:F1D26

Project description:Streptomyces clavuligerus strain:F1D26 Genome sequencing

| PRJNA679972 | ENA

Streptomyces clavuligerus strain:F1D10

Project description:Streptomyces clavuligerus strain:F1D10 Genome sequencing