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Gene expression data of murine hematopoietic stem cells after in vitro culture and anemic stress

ABSTRACT: Gene expression data of murine hematopoietic stem cells after in vitro culture and anemic stress

PROVIDER: PRJNA681809 | ENA |

REPOSITORIES: ENA

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Gene expression data of murine hematopoietic stem cells after in vitro culture and anemic stress

Project description:Isolation of long-term reconstituting hematopoietic stem cell (HSC) has been possible by utilizing flow cytometry with a combination of multiple antibodies against cell surface markers. However those cell surface phenotypes do not represent functional HSC after in vitro culture. We compared gene expression profiling of phenotypic HSC (CD48-KSL cells) before and after in vitro culture, and discovered that cultured HSC express mast cell-related genes including Cd244. After in vitro culture, phenotypic HSC can be divided into CD244- and CD244+ subpopulations, and only CD244- cells that have low mast cell gene expression and maintain HSC-related genes sustain reconstitution potential. Induction of CD244 may partially be triggered by induction of endoplasmic reticulum (ER) stress, as chemically induced ER stress signal increased CD244+ subpopulation while ER stress suppression using a molecular chaperone, TUDCA, decreased CD244+ population, which were correlated to the output of reconstitution assay. These data suggest CD244 positivity is a potent marker to exclude non-functional HSC after in vitro culture thereby useful to elucidate mechanism of functional decline of HSC during ex vivo treatment. It has been unknown whether HSC respond to acute anemic stress. We compared frequency and differentiation potential of HSC in steady-state vs acute anemic mice, and discovered that HSC rapidly expand upon the anemia induction and change their lineage balance to more erythrocytic.

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