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Differential expression of genes in roots of rice seedlings with contrasting response under low nitrogen

ABSTRACT: Differential expression of genes in roots of rice seedlings with contrasting response under low nitrogen

PROVIDER: PRJNA681819 | ENA |

REPOSITORIES: ENA

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Differential expression of genes in shoot samples of rice seedlings with contrasting response under low nitrogen

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Differential expression of genes in roots of rice seedlings with contrasting response under low nitrogen

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Differential expression of genes in shoot samples of rice seedlings with contrasting response under low nitrogen

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Root aeration improves growth and nitrogen accumulation in rice seedlings under low nitrogen.

Project description:In wetland soils, changes in oxygen (O2) level in the rhizosphere are believed to influence the behaviour of nutrients and their usage by plants. However, the effect of aeration on nitrogen (N) acquisition under different N supply conditions remains largely unknown. In this study, the rice cultivars Yangdao 6 (YD6, with higher root aerenchyma abundance) and Nongken 57 (NK57, with lower root aerenchyma abundance) were used to evaluate the effects of aeration on rice growth and N accumulation. Our results showed that the number of adventitious roots and the root surface area increased significantly, and ethylene production and aerenchyma formation decreased in both cultivars after external aeration (EA). Five N treatments, including no N (-N), 0.125 mM NH4NO3 (LN), 1.25 mM Ca(NO3)2 (NO3-N), 1.25 mM (NH4)2SO4 (NH4-N) and 1.25 mM NH4NO3 (N/N), were applied to YD6 and NK57 for 2 days under internal aeration or EA conditions. External aeration increased the root biomass in both cultivars and the shoot biomass in NK57 by 18-50 %. The total N concentrations in roots of YD6 grown under -N and LN and of NK57 grown under NO3-N were increased by EA. Expression of OsPAD4, one of four putative genes regulating aerenchyma formation, showed a similar pattern alongside changes in the ethylene level in the EA-treated rice irrespective of the N treatments. Furthermore, expression of the high-affinity nitrate transporter gene OsNRT2.1 was increased by EA under -N, LN and NO3-N conditions. Our data provide evidence of an interaction between O2 and the supply of N in ethylene production, aerenchyma formation and N nutrition through modification of the expression of OsPAD4 and OsNRT2.1.

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Drought responsive transcriptome profiling in roots of contrasting rice genotypes IR20 and Nootripathu

Project description:Drought avoidance mechanism is one of the component mechanisms contributing for drought tolerance in which roots serves as the master keys, but poorly understood. Comparative analysis of drought stress responsive root transcriptome between drought-tolerant Nootripathu and drought-susceptible IR20 In this study, we used microarrays to dissect out drought responsive changes in roots of two contrasting rice genotypes viz., IR 20 (a shallow rooted lowland indica genotype) and Nootripathu (a deep rooted upland indica genotype) at molecular level.

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