Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile of AT1-like cells in monoculture or cocultured with control or shEphB6-depleted D2.0R-EGFP cells

ABSTRACT: Gene expression profile of AT1-like cells in monoculture or cocultured with control or shEphB6-depleted D2.0R-EGFP cells

PROVIDER: PRJNA681868 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13176216.fastq.gz	Fastqsanger.gz
	SRR13176217.fastq.gz	Fastqsanger.gz
	SRR13176218.fastq.gz	Fastqsanger.gz
	SRR13176219.fastq.gz	Fastqsanger.gz
	SRR13176220.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Gene expression profile of control or shEphB6-depleted D2.0R-EGFP cells in monoculture or cocultured with AT1-like cells

Project description:Gene expression profile of control or shEphB6-depleted D2.0R-EGFP cells in monoculture or cocultured with AT1-like cells

| PRJNA681867 | ENA

Gene expression profile of control or shEphB6-depleted D2.0R-EGFP cells in monoculture or cocultured with AT1-like cells

Project description:We studied how crosstalk with AT1-like cells affects transcriptome of disseminated dormant breast cancer cells and the requirement of EphB6 expression in the cancer cells for this genetic program

2021-03-04 | GSE162439 | GEO

Gene expression profile of AT1-like cells in monoculture or cocultured with control or shEphB6-depleted D2.0R-EGFP cells

Project description:We studied how crosstalk with disseminated dormant breast cancer cells affects transcriptome of AT1-like cells and the requirement of EphB6 expression in the cancer cells for this genetic program

2021-03-04 | GSE162438 | GEO

Tgfb in AT1 cells

Project description:Premature birth disrupts normal lung development and places infants at risk for bronchopulmonary dysplasia (BPD), a disease increasing in incidence which disrupts lung health throughout the lifespan. The TGFβ superfamily has been implicated in BPD pathogenesis, however, what cell lineage it impacts remains unclear. We show that the primary TGFβ receptor, Tgfbr2, is critical for AT1 cell fate maintenance and function. Loss of Tgfbr2 in AT1 cells during late lung development leads to AT1-AT2 cell reprogramming and altered pulmonary architecture, which persists into adulthood. Restriction of fetal lung stretch and associated AT1 cell spreading through a model of oligohydramnios enhances AT1-AT2 reprogramming. Transcriptomic and proteomic analysis reveal that expression of extracellular matrix components by AT1 cells is attenuated with loss of Tgfbr2. Cell spreading assays shows that TGFβ signaling regulates integrin transcription to alter AT1 cell morphology, which further impacts ECM expression through changes in mechanotransduction. These data reveal the cell intrinsic necessity of TGFβ signaling to maintain AT1 fate and define this cell lineage as a major orchestrator of the alveolar matrisome, which, if altered, may predispose to BPD.

2024-03-04 | GSE230268 | GEO

Gene expression profile of EphB6-depleted D2.0R-EGFP cells in vivo

Project description:Gene expression profile of EphB6-depleted D2.0R-EGFP cells in vivo

| PRJNA681866 | ENA

Tgfb in AT1 cells

Project description:Tgfb in AT1 cells

| PRJNA958167 | ENA

Bradyrhizobium sp. AT1

Project description:Bradyrhizobium sp. AT1 Genome sequencing and assembly

| PRJNA271718 | ENA

DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model

Project description:In this study, we constructed a tissue-engineered DRG-CSC (corneal stromal cells) co-cultured model (DS model), a CSC monoculture model (S model), and a DRG monoculture model (D model). Chicken DRGs were extracted from E7-E10 chicken embryo. Human CSCs were extracted in stromal lenticules from small incision lenticule extraction (SMILE). Transcriptional profiling of DRG in the DS model (DS-D), DRG in the D model(D-D), CSCs in the DS model (DS-S), CSCs in the S model (S-S) was analyzed. After 7 days of culture, RNA of each group was extracted.

2021-06-29 | GSE179020 | GEO

N-Acetylation of secreted proteins is widespread in Apicomplexa and independent of acetyl-CoA transporter AT1

Project description:Acetyl-CoA critically participates in post-translational modification of proteins, central carbon and lipid metabolism in several compartments of eukaryotic cells. In mammals, the acetyl-CoA transporter 1 (AT1) facilitates the flux of cytosolic acetyl-CoA into the endoplasmic reticulum (ER) enabling the acetylation of proteins of the secretory pathway in concert with dedicated acetyltransferases including Nat8. Homologues of AT1 and Nat8 were identified in the apicomplexan parasites Toxoplasma gondii and Plasmodium berghei. However, the implication of acetyl-CoA pool influx into the ER in acetylation of ER-transiting proteins and their relevance throughout the parasites’ life cycle is unknown. Here, we report proteome-wide analyses, which revealed unprecedented widespread N-terminal acetylation marks of secreted proteins in both parasites. Deletion of the gene coding for AT1 in both parasites, resulted in global loss of fitness and in addition to retardation in erythrocytic development, malaria parasites are blocked in transmission to mosquitoes. However, in absence of AT1 proteome wide lysine and N-terminal acetylation modifications remain unaltered. This highlights a role of AT1 in parasite development uncoupled to acetylation capacity, indicative of an unusually active acetylation machinery occurring in the ER of Apicomplexa.

2022-06-20 | PXD029616 | Pride

Methanimicrococcus hacksteinii strain:At1

Project description:Methanimicrococcus hacksteinii strain:At1 Genome sequencing and assembly

| PRJNA983583 | ENA