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Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

ABSTRACT: Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

PROVIDER: PRJNA682179 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13183000.fastq.gz	Fastqsanger.gz
	SRR13183001.fastq.gz	Fastqsanger.gz
	SRR13183002.fastq.gz	Fastqsanger.gz
	SRR13183003.fastq.gz	Fastqsanger.gz
	SRR13183004.fastq.gz	Fastqsanger.gz

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Similar Datasets

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [degradome]

Project description:Soil potassium deficiency has become a global problem in agricultural production, seriously restricting crops productions and agricultural sustainable development. Identification of the microRNAs and understanding their functions in response to low K stress will be helpful for developing crop varieties with low K tolerance. Our previous study identified a low K tolerant accession XZ153 from Tibetan wild barley. In this study, small RNA and degradome analysis were performed on two barley genotypes differing in low K tolerance (XZ153, tolerant; ZD9, sensitive) to identify the miRNAs and their targets responding to low K stress. A total of 1108 miRNAs were detected in shoots of XZ153, and ZD9 at 2 d and 7 d after low K stress, and their targets were identified through bioinformatics prediction and degradome analysis. We identified 65 differentially expressed miRNAs responding to low K stress. The results also showed that miR164c, miR169h and miR395a modules could mediate TCA cycle, glycolysis pathway and pentose phosphate pathway responding to low K stress. The osa-miR166g-3p and ghr-miR482b may act as the regulators in Ca2+ signaling pathway in response to low K stress. The methionine salvage cycle involved in ethylene biosynthesis process mediated by miR396c-3p and osa-miR171e-5p might be also involved in responding to low K stress. Some miRNAs, including miR160a, miR396c and miR169h modules, which participated in photosynthesis regulation under low K stress, differed between the two barley genotypes. In conclusion, these exclusively expressed miRNAs and their targets play the crucial roles in low K tolerance.

2020-12-06 | GSE162540 | GEO

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

2020-12-06 | GSE162539 | GEO

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley

Project description:Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley

| PRJNA682173 | ENA

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [degradome]

Project description:Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [degradome]

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Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-06 | GSE162541 | GEO

H3K27me3 ChIP-seq on 16 days after pollination endosperm of cultivated barley

Project description:To provide comprehensive spatiotemporal information about biological processes in developing grains of cultivated barley (Hordeum vulgare subsp. vulgare), we performed a chromatin immunoprecipitation of H3K27me3 followed by high-throughput sequencing (ChIP-seq) in barley endosperm at 16 days after pollination.

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Project description:Tibetan hulless barley (Hordeum vulgare L. var. nudum) is one of the primary crops cultivated in the mountains of Tibet suffering from low temperature, high salinity, and drought. Specifically, drought is one of the major abiotic stresses that affect and limit Tibetan barley growth. Thus, it's critical to explore the molecular mechanism of hulless barley during arid or drought environmental conditions for improving crop yield. Here, we employed quantitative proteomics by data-independent acquisition mass spectrometry (DIA-MS) to investigate protein expression in tolerant (XL) and sensitive (DQ) cultivars.

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Deep-sequencing of degraded or cleaved RNAs from barley shoot and root growing in low-Pi condition.

Project description:RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries.

2021-02-17 | GSE145423 | GEO

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