Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in the fly and mouse

ABSTRACT: Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in the fly and mouse

PROVIDER: PRJNA682340 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13190128_1.fastq.gz	Fastqsanger.gz
	SRR13190128_2.fastq.gz	Fastqsanger.gz
	SRR13190129_1.fastq.gz	Fastqsanger.gz
	SRR13190129_2.fastq.gz	Fastqsanger.gz
	SRR13190130_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in the fly and mouse

Project description:Identification of Gsx2 genomic binding patterns from E12.5 mouse lateral ganglionic eminence (LGEs).

2020-12-04 | GSE162589 | GEO

Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in flies and mice.

Project description:How homeodomain proteins gain sufficient specificity to control different cell fates has been a long-standing problem in developmental biology. The conserved Gsx homeodomain proteins regulate specific aspects of neural development in animals from flies to mammals, and yet they belong to a large transcription factor family that bind nearly identical DNA sequences in vitro. Here, we show that the mouse and fly Gsx factors unexpectedly gain DNA binding specificity by forming cooperative homodimers on precisely spaced and oriented DNA sites. High-resolution genomic binding assays revealed that Gsx2 binds both monomer and homodimer sites in the developing mouse ventral telencephalon. Importantly, reporter assays showed that Gsx2 mediates opposing outcomes in a DNA binding site-dependent manner: Monomer Gsx2 binding represses transcription, whereas homodimer binding stimulates gene expression. In Drosophila, the Gsx homolog, Ind, similarly represses or stimulates transcription in a site-dependent manner via an autoregulatory enhancer containing a combination of monomer and homodimer sites. Integrating these findings, we test a model showing how the homodimer to monomer site ratio and the Gsx protein levels defines gene up-regulation versus down-regulation. Altogether, these data serve as a new paradigm for how cooperative homeodomain transcription factor binding can increase target specificity and alter regulatory outcomes.

| S-EPMC7778271 | biostudies-literature

Comparison of IND induced vs. mock treated seedlings: Transcriptome profiling upon induction of the transcription factor IND

Project description:rs10-03_ind - comparison of ind induced vs mock treated seedlings - Identification of miRNAs regulated by IND - Transgenic seeds containing an IND transgene under the control of a dexamethasone inducible promoter were germinated and grown in liquid Murashige/Skoog medium under a 16h light/8h darkness light regime. After seven days of growth, seedlings were either mock-treated or treated with 10 micromolar dexamethasone for 24 hours.

2011-05-12 | GSE28898 | GEO

Comparison of IND induced vs. mock treated seedlings: Transcriptome profiling upon induction of the transcription factor IND

2011-05-12 | E-GEOD-28898 | biostudies-arrayexpress

Expression data from wild and ind-6 mutant Arabidopsis silique during three development stages of fruit dehiscence

Project description:Fruit dehiscence is an essential developmental process of some economic crops that impacts dramatically on crop yields and involves altered regulation of thousands of genes, ind-6 gene is a transcription factor play important role during fruit dehiscence. Gene expression were examined in wild-type and ind-6 mutants at three development stages of fruit dehiscence to explore the molecular mechanism of ind-6 in fruit dehiscence. We used microarrays to examine changes in gene expression during fruit dehiscence and identified distinct classes of up- and down-regulated genes in wild-type and ind-6 mutant genotypes.

2015-12-28 | GSE66672 | GEO

Transcriptome analysis of genes regulated by INDEHISCENT (IND) transcription factor under the control of the 35S promoter after treatment with auxin

Project description:IND is a regulator of fruit development in Arabidopsis thaliana. To identify genes regulated by IND we performed array based transcriptome analysis of Dexamethasone (DEX) inducible IND transgenic seedlings. One week old 35S::IND:GR seedlings were treated with DMSO, Auxin, DEX and Auxin plus DEX for 6 h. Three biological independent experiments were performed.

2017-07-05 | E-MTAB-3812 | biostudies-arrayexpress

Hydra vulgaris strain:Ind-Pune

Project description:Hydra vulgaris strain:Ind-Pune Epigenomics

| PRJNA532677 | ENA

Modulation of apple leaf trancriptome by tomato cutin monomer extract

Project description:Investigation of whole genome gene expression level changes in leaves of apple seedlings (Golden delicious) 3 days after treatment by tomato cutin monomer extract (CME) versus formulation blank (FB). CME is a formulated extract enriched of hydroxy fatty acids from tomato cuticle

2024-02-09 | GSE245564 | GEO

Hydra vulgaris strain:Ind-Pune

Project description:Hydra vulgaris strain:Ind-Pune Transcriptome or Gene expression

| PRJNA451264 | ENA

Saccharum spontaneum cultivar:IND 81 013

Project description:Saccharum spontaneum IND 81 013 Resequencing genome sequencing

| PRJNA651919 | ENA