Trichechus manatus latirostris strain:Wild Caught
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA68243 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR307134_1.fastq.gz | Fastqsanger.gz | |||
| SRR307134_2.fastq.gz | Fastqsanger.gz | |||
| SRR307135_1.fastq.gz | Fastqsanger.gz | |||
| SRR307135_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 69 |
Similar Datasets
Project description:Trichechus manatus latirostris Organism overview
| PRJNA242269 | ENA
Project description:Trichechus manatus latirostris Genome sequencing and assembly
| PRJNA939417 | ENA
Project description:Investigating the Gene Expression Profiles of Florida Manatees (Trichechus manatus latirostris) Rehabilitated Following a Red Tide
| PRJNA342504 | ENA
Project description:To investigate a Florida manatee (Trichechus manatus latirostris) mortality event following a red tide bloom in Southwest Florida, a RNA-Seq experiment was conducted. Cell processes such as immune response, cell proliferation and differentiation and apoptosis were among the most affected by red tide. These were involved in potential diseases such as neoplasms, inflammation, and wounds and injuries, among others. There were both up-regulated and down-regulated genes, but the highest fold changes relative to controls were for genes that were down-regulated. Piccolo presynaptic cytomatrix protein (PCLO) gene, the one most down-regulated (fold change -9.93; p-value 0.0009) is associated with neurotransmitter release, cognitive functioning, neuronal loss, and neuronal synapse function. Another gene that has a similar function, ankyrin 2, neuronal, transcript variant 1 (ANK2) was also down-regulated (fold change -8.66; p-value 0.0023). ANK2 is associated with the stability of neuron synapses. Two immune genes, interleukin 6 (interferon, beta 2) (IL6) and zinc finger protein 804B (ZNF804B), were down-regulated (fold change -9.31; p-value 0.000003 and fold change -8.90; p-value 0.0164, respectively). Interleukin 6 encodes proteins involved in acute phase response, inflammation, and autoimmune response. ZNF804B is associated with neuronal chemokine and cytokine regulation, autoimmune response, and immune activation. The family with sequence similarity 186, member A (FAM186A) gene was down-regulated (fold change -8.79; p-value 0.0143). FAM186A gene mutation is associated with tumor metastasis in colorectal cancer tumors. Among the most up-regulated genes, CCAAT/enhancer binding protein (C/EBP) is involved in granulocytic differentiation and also involved with the immune system. Determining the differentially expressed genes associated with red tide enhances our understanding of manatee immune response to red tide toxins and aids in the development of red tide biomarkers. This information will better assist clinicians and researchers in diagnosing and treating future illnesses.
2017-10-01 | GSE86792 | GEO
Project description:The Florida manatee (Trichechus manatus latirostris Linnaeus 1758) actively selects and consumes the "chicken-liver" sponge Chondrilla caribensis. Manatees ate over 10% of C. caribensis on a sample dock, mostly from pylons that received no direct sunlight. Since manatees reportedly eat mostly seagrasses and algae, it was thought that the chlorophyll-a content of the symbiotic cyanobacteria in C. caribensis might be correlated to the amount eaten; however the correlation was not significant (P > 0.05). C. caribensis has variable chemical defenses and round spherasters (spicules), but these do not appear to be effective deterrents to predation by manatees. This is the first direct evidence that manatees actively seek out and consume a sponge.
| S-EPMC6982412 | biostudies-literature
Project description:A survey for the presence of nematodes on the skin of the native Florida manatee, Trichechus manatus latirostris from Crystal River, Florida was conducted during annual manatee health assessments. A putative isolate of Cutidiplogaster manati (Diplogastridae) and two other nematodes belonging to the same family were recovered from mid-dorsal tail skin-scrapings from all sampled winter-collected healthy wild adult manatees during two successive years (2018-2019). Qualitative abundance estimates of these three species of diplogastrid nematodes suggest that an average wild Florida manatee adult might possess between 30,000 and 120,000 nematodes on its tail dorsum and that the entire body dorsum including the tail might possess 160,000-640,000 nematodes in roughly equal ratios. Attempts to culture these nematodes on a variety of different culture media were unsuccessful but examination of the mouth (stomatal) morphology suggests specialized feeding on microbes such as diatoms or predation on other nematodes. No skin lesions were observed during the 2018-2019 samplings suggesting that under normal conditions these nematodes are highly specialized free-living epibionts of the skin that are tightly bound to this niche and horizontally transferred between individual manatees in an analogous fashion to human skin mites (Demodex folliculorum and D. brevis). Molecular phylogenetic inferences using sequences of near full length SSU and D2-D3 expansion segments of LSU rRNA genes revealed a putative new morphospecies in Cutidiplogaster sister to C. manati that was monophyletic with several named Mononchoides species, and another putative new morphospecies that formed a clade with several undescribed species similar in appearance to Mononchoides as well as Tylopharyx, Eudiplogasterium, Paroigolaimella and Sachsia.
| S-EPMC7806751 | biostudies-literature
Project description:A retrospective study of admission data of 401 West Indian manatees (Trichechus manatus latirostris) presented to the David A. Straz Jr. Manatee Critical Care Center at ZooTampa at Lowry Park (ZooTampa) for rehabilitation from August 1991 through October 2017. Causes of admittance, location of rescue, gender, and age class were all recorded for each manatee admitted. Admittance categories as defined by the Florida Fish and Wildlife Conservation Commission (FWC) included watercraft collisions, natural causes, entanglement, entrapment, orphaned calves, captive born, mothers of rescued calves, calves of rescued mothers, human, and other. The admitted population was primarily from the southwest and northwest coasts and related waterways of Florida. The gender difference was relatively equivocal (54% female) while the adults comprised 79% of the admissions. The overall total admissions increased steadily over the study period as did the admissions for each individual categories of admission. Watercraft collisions and natural causes combined were 71% of all admissions for the entire study period and are the dominant causes of admission. Watercraft collisions are more likely to occur during May through October, whereas natural causes of admittance are more likely to occur between December and March. Rehabilitated manatees may reduce overall manatee mortality and can provide insight into population-based health concerns if evaluated appropriately. Future efforts can incorporate physical examination findings, hematology, biochemistry profiles, and ancillary diagnostic testing to continue to improve the individual welfare of this marine mammal in its natural range. Admissions data could also potentially serve the wider conservation and recovery efforts if it is proven that the data obtained is at least as informative as that obtained by the carcass salvage program. Limited conservation resources could then be re-directed as new challenges arise with the expanding population and potentially expanding range of this species.
| S-EPMC7347118 | biostudies-literature
Project description:To investigate a Florida manatee (Trichechus manatus latirostris) mortality event following a red tide bloom in Southwest Florida, an RNA sequencing experiment was conducted. Gene expression changes in white blood cells were assessed in manatees rescued from a red tide affected area (n = 4) and a control group (n = 7) using RNA sequencing. The genes with the largest fold changes were compared between the two groups to identify molecular pathways related to cellular and disease processes. In total, 591 genes (false discovery rate <0.05) were differentially expressed in the red tide group. Of these, 158 were upregulated and 433 were downregulated. This suggests major changes in white blood cell composition following an exposure to red tide. The most highly upregulated gene, Osteoclast associated 2C immunoglobulin-like receptor (OSCAR), was upregulated 12-fold. This gene is involved in initiating the immune response and maintaining a role in adaptive and innate immunity. The most highly downregulated gene, Piccolo presynaptic cytomatrix protein (PCLO), was downregulated by a factor of 977-fold. This gene is associated with cognitive functioning and neurotransmitter release. Downregulation of this gene in other studies was associated with neuronal loss and neuron synapse dysfunction. Among the cellular pathways that were most affected, immune response, including inflammation, wounds and injuries, cell proliferation, and apoptosis were the most predominant. The pathway with the most differentially expressed genes was the immune response pathway with 98 genes involved, many of them downregulated. Assessing the changes in gene expression associated with red tide exposure enhances our understanding of manatee immune response to the red tide toxins and will aid in the development of red tide biomarkers.
| S-EPMC7331979 | biostudies-literature
Project description:The recognized impact of parasites in wildlife populations demands surveillance of endangered species like the Antillean manatees (Trichechus manatus manatus) in Colombia. We conducted a parasitological survey in four rescued sea cows in order to document the parasite diversity of this sirenian in the Caribbean wetland of Colombia and contribute to the molecular characterization of its trematodes. The flukes Chiorchis fabaceus, Nudacotyle undicola and the protozoans Eimeria manatus and E. nodulosa were identified in analysed faecal samples. For C. fabaceus and N. undicola, partial regions of ribosomal RNA genes were amplified and sequenced in order to infer their phylogenetic relations. The current study constitutes a new sirenian host (T. manatus manatus) record for the genus Eimeria and the trematode N. undicola.
| S-EPMC6031961 | biostudies-literature