Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Escherichia coli O104:H4

ABSTRACT: Escherichia coli O104:H4 outbreak

PROVIDER: PRJNA68275 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	AFOB02.dat.gz	Other
	AFOB02.fasta.gz	Fasta.gz
	AFOB02.master.dat	Other
	AFOG01.dat.gz	Other

Items per page:

1 - 5 of 437

Similar Datasets

RpoS acts as a global repressor of virulence gene expression in Escherichia coli O104:H4 and enteroaggregative E. coli

Project description:In 2011, in Germany, Escherichia coli O104:H4 caused the enterohemorrhagic E. coli (EHEC) outbreak with the highest incidence rate of hemolytic uremic syndrome. This pathogen carries an exceptionally potent combination of EHEC- and enteroaggregative E. coli (EAEC)-specific virulence factors. Here, we identified an E. coli O104:H4 isolate that carried a single nucleotide polymorphism (SNP) in the start codon (ATG>ATA) of rpoS, encoding the alternative sigma factor S. The rpoS ATG>ATA SNP was associated with enhanced EAEC-specific virulence gene expression. Deletion of rpoS in E. coli O104:H4 Dstx2 and typical EAEC resulted in a similar effect. Both rpoS ATG>ATA and DrpoS strains exhibited stronger virulence-related phenotypes in comparison to wild type. Using promoter-reporter gene fusions, we demonstrated that wild-type RpoS repressed aggR, encoding the main regulator of EAEC virulence. In summary, our work demonstrates that RpoS acts as a global repressor of E. coli O104:H4 virulence, primarily through an AggR-dependent mechanism.

2024-04-04 | GSE243699 | GEO

Differential RNA-Seq (dRNA-seq) of Escherichia coli O104:H4

Project description:We performed a high-throughput mapping of the 5’ end transcriptome of the pAA plasmid of the clinical Escherichia coli O104:H4 (E. coli O104:H4) isolate LB226692. We employed differential RNA-sequencing (dRNA-seq), a terminator exonuclease (TEX)-based RNA-seq approach allowing for the discrimination of primary and processed transcripts. This method has proven to be a powerful tool for the mapping of transcription start sites (TSS) and detection of non-coding RNAs (ncRNAs) in bacteria. We catalogued pAA-associated TSS and processing sites on a plasmid-wide scale and performed a detailed analysis of the primary transcriptome focusing on pAA virulence gene expression.

2016-10-19 | GSE78041 | GEO

Escherichia coli O104:H4

Project description:Escherichia coli O104:H4

| PRJEB49642 | ENA

RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens

Project description:Here, we investigated the impact of Stx2 phage carriage on Escherichia coli (E. coli) K-12 MG1655 host gene expression. Using quantitative RNA-seq analysis, we compared the transcriptome of naïve MG1655 and the lysogens carrying the Stx2 phage of the 2011 E. coli O104:H4 outbreak strain or of the E. coli O157:H7 strain PA8, which share high degree of sequence similarity.

2019-06-17 | GSE126710 | GEO

Escherichia coli O104:H4

Project description:Differential RNA-Seq (dRNA-seq) of Escherichia coli O104:H4

| PRJNA312402 | ENA

Escherichia coli O104:H4 strain:LB226692

Project description:Escherichia coli O104:H4 strain:LB226692 Genome sequencing

| PRJNA419526 | ENA

Escherichia coli O104:H4 str. H112180541

Project description:Escherichia coli O104:H4 str. H112180541 genome sequencing

| PRJNA68163 | ENA

Escherichia coli O104:H4 str. ON2011

Project description:Escherichia coli O104:H4 str. ON2011 Genome sequencing and assembly

| PRJNA81623 | ENA

Escherichia coli O104:H4 str. H112180282

Project description:Escherichia coli O104:H4 str. H112180282 Genome sequencing and assembly

| PRJNA68661 | ENA

Escherichia coli O104:H4 str. GOS1

Project description:Escherichia coli O104:H4 str. GOS1 Genome sequencing and assembly

| PRJNA71263 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data