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The PAF1 complex promotes 3‘ processing of pervasive transcripts [ChIP-seq]

ABSTRACT: The PAF1 complex promotes 3‘ processing of pervasive transcripts [ChIP-seq]

PROVIDER: PRJNA683577 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13272600_1.fastq.gz	Fastqsanger.gz
	SRR13272600_2.fastq.gz	Fastqsanger.gz
	SRR13272601_1.fastq.gz	Fastqsanger.gz
	SRR13272601_2.fastq.gz	Fastqsanger.gz
	SRR13272602_1.fastq.gz	Fastqsanger.gz

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The PAF1 complex promotes 3‘ processing of pervasive transcripts

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The PAF1 complex promotes 3‘ processing of pervasive transcripts [PRO-Seq]

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The PAF1 complex promotes 3‘ processing of pervasive transcripts [ChIP-seq]

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The PAF1 complex promotes 3‘ processing of pervasive transcripts

Project description:The PAF1 complex (PAF1C) functions in multiple transcriptional processes involving RNA Polymerase II (Pol II). eRNAs and PROMPTs are pervasive transcripts transcribed by Pol II and rapidly degraded by the nuclear exosome complex after 3’ endonucleolytic cleavage by the Integrator complex (Integrator). Here we show that PAF1C has an unexpected role in the termination of eRNAs and PROMPTs that are cleaved 1-3 kb downstream of the transcription start site. Mechanistically, PAF1C facilitates recruitment of Integrator to sites of pervasive transcript cleavage, promoting timely cleavage and transcription termination. We also show that PAF1C recruits Integrator to coding genes, where PAF1C then dissociates from Integrator upon entry into processive elongation. Our results demonstrate an unexpected function for PAF1C in limiting the length and accumulation of pervasive transcripts that result from nonproductive transcription

2022-03-03 | PXD031531 | Pride

The PAF1 complex promotes 3‘ processing of pervasive transcripts [RNA-seq]

Project description:The PAF1 complex promotes 3‘ processing of pervasive transcripts [RNA-seq]

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Project description:The PAF1 complex promotes 3‘ processing of pervasive transcripts [3'-seq]

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Project description:The PAF1 complex promotes 3‘ processing of pervasive transcripts [PRO-Seq]

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Project description:To identify the direct targets of the Paf1/RNA polymerase II complex we compared expression profiles of isogenic wild type and paf1 and ctr9 mutant strains. We also created a Tet-regulated form of Paf1 and monitored expression patterns after shut off of Paf1. Samples were isolated at one hour intervals from 1 to 8 hours after shut off. Experiment Overall Design: Transcripts were compared on Affymetrix microarrays using standard protocols.

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