Altered thymic selection in Themis-deficient mice leads to expansion of an unusual virtual memory and bystander-competent CD8+ subpopulation bearing Va3.2 TCR
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA683938 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR13234365_1.fastq.gz | Fastqsanger.gz | |||
| SRR13234365_2.fastq.gz | Fastqsanger.gz | |||
| SRR13234366_1.fastq.gz | Fastqsanger.gz | |||
| SRR13234366_2.fastq.gz | Fastqsanger.gz | |||
| SRR13234367_1.fastq.gz | Fastqsanger.gz |
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Project description:This study aimed to compare the TCRab repertoires from CD8 T cell subsets in a different T cell progression stage in Themis-sufficient and Themis-deficient mice. Analysis of the NGS-derived data revealed an overall reduction of the TCRa repertoire diversity in Themis's absence. In contrast, the CD8 compartment bearing the Va3.2 TCRs in the Themis-deficient model retains higher diversity than the repertoire from the Themis-sufficient counterpart. Furthermore, analysis of CDR3 peptide sequences of Va3.2+ and Va3.2- TCRs disclose the greater contribution of the hydrophobic residues in the former repertoires, indicating enhanced self-reactivity within this CD8+ T cell fraction.
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