Project description:To investigate the differently expressed genes treated with DMSO, extracts of Rhizoma Coptidis and its main alkoids berberine, coptisine and palmatine
Project description:We explored whether an ethanol extract of coptidis rhizoma (ECR) exhibited anti-teratoma activity and identified the main active components involved in the selective elimination of undifferentiated iPSCs.
Project description:The anti-diabetes activity of Rhizoma Coptidis was documented in the book âNotes of Elite Physiciansâ written by Hongjing Tao 1500 years ago. Berberine (BBR) is the major isoquinoline alkaloid constituent of the Chinese herb Rhizoma Coptidis, which has the beneficial characteristic of regulating glucose and lipid metabolism and has been extensively used in the treatment of obesity, diabetes and hypercholesterolemia. However, the effect of berberine on specific diabetes treatment targets is unknown. This study was conducted to elucidate the genome-wide gene expression profile in Zucker Diabetic Fatty Rats liver tissues in response to Berberine treatment.
Project description:An ethanol extract of Coptidis rhizoma induces apoptotic cell death in induced pluripotent stem cells and suppress teratoma formation
Project description:Rhizoma coptidis (R.coptidis) is a widely cultivated traditional Chinese herb. Although the chemical profiles of R.coptidis have been established previously, the biological profiling of R.coptidis has not been conducted yet. Here, we collected R.coptidis varieties from four different regions as well as its analogue, Rabdosia serra (R.serra) were and performed genome-wide biological response fingerprinting (BioReF) on HepG2 cells using a gene expression array.
Project description:This study aimed to investigate the mechanism of C2S-induced macrophagic inflammation and its’ effect on osteogenic differentiation of precursor cells. This study used the C2S (75-150 μg/ml) extract to induce macrophagic inflammation in RAW264.7 murine macrophages. RNA sequencing was preformed for analyzing mechanism of mitochondrial function and autophagy.
Project description:To investigate the toxicity of long-term treatment with Asari Radix et Rhizoma on hematopoietic cells, we have performed the 28 days gavage administration of AR in C57BL/6 mouse. Then, we have harvested the RBC lysed bone marrow cells, and analyzed the RNA-seq. analysis.