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Comparison of E14.5 embryonic kidney transcriptomes (Grb2 ureteric-specific knockout vs wild-type)

ABSTRACT: Comparison of E14.5 embryonic kidney transcriptomes (Grb2 ureteric-specific knockout vs wild-type)

PROVIDER: PRJNA684494 | ENA |

REPOSITORIES: ENA

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Comparison of E14.5 embryonic kidney transcriptomes (Grb2 ureteric-specific knockout vs wild-type)

Project description:Grb2 is a small SH2-SH3 adaptor molecule that interacts with activated tyrosine kinase receptors (RTKs), providing a crucial link towards downstream activation of pro-proliferative and pro-survival ERK and Akt signaling pathways. Ret and FGFR2, two RTKs that interact with Grb2, play important roles in ureteric branching and the proper establishment of the renal collecting duct system, but it is unclear whether Grb2 is required in this process. In this study, we selectively ablated a conditional floxed allele of the Grb2 gene within the ureteric epithelial lineage in mice and demonstrate that Grb2 signaling is essentially required for proper collecting duct development. Ureteric Grb2 deficiency results in perinatal lethality and severe renal hypodysplasia closely reminiscent of the rudimentary kidney phenotypes observed in Ret-null mutant mice. Grb2 loss attenuates ERK and Akt activation in the ureteric epithelia resulting in pronounced impairment of ureteric branching. Gene expression analysis reveals that Grb2 deficiency results in defective induction of genes implicated in both ureteric branching and reciprocal mesenchymal metanephric induction. Our findings therefore strongly indicate that Grb2 is a physiologically-relevant major RTK signaling relay partner that promotes renal branching morphogenesis and the proper development of the collecting duct system and the urogenital tract. Microarray was used to profile and compare the transcriptomes of developing kidneys of E14.5 Grb2 conditional knockout (ureteric-bud specific) and wild-type embryos

2023-12-01 | GSE163040 | GEO

Hepatocyte nuclear factor 1β gene regulation in E14.5 ureteric bud cells

Project description:HNF-1β mutations are one of the most common single-gene mutations that underlie kidney developmental disease. Hepatocyte nuclear factor 1β (HNF-1β) is essential for kidney development, but its functions in ureteric bud (UB) branching morphogenesis are incompletely understood. We isolated E14.5 UB cells using fluorescence-activated cell sorting, and performed RNA-sequencing to compare gene expression in wild-type and HNF-1β deficient UB cells. 1632 genes have significantly lower expression in the HNF-1β deficient UB cells and 2223 genes have significantly higher expression in HNF-1β deficient UB cells.

2022-10-20 | GSE205217 | GEO

Hepatocyte nuclear factor 1β gene regulation in E14.5 ureteric bud cells

Project description:Hepatocyte nuclear factor 1β gene regulation in E14.5 ureteric bud cells

| PRJNA844104 | ENA

Generation of kidney ureteric bud and collecting duct organoid

Project description:A platform for generating expandable, branching and gene-editable ureteric bud organoid from primary mouse and human ureteric bud progenitor cells and human pluripotent stem cells, and its maturation into collecting duct organoid.

2021-05-11 | GSE149109 | GEO

Transcripts identified by microarray analysis that were deregulated in E14.5 Tbx18cre/+;Gata2fl/fl (Gata2cKO) ureters

Project description:Evaluation of the transcriptional changes in E14.5 ureters upon loss of Gata2 in the ureteric mesenchyme.

2020-12-31 | GSE127702 | GEO

Transcripts identified by microarray analysis that were deregulated in E14.5 Tbx18(cre/+);Gata6(fl/fl) (Gata6cKO) ureters

Project description:Evaluation of the transcriptional changes in E14.5 ureters upon loss of Gata6 in the ureteric mesenchyme.

2022-01-04 | GSE174614 | GEO

Transcripts identified by microarray analysis that were deregulated in E14.5 Tbx18(cre/+);Rbpj(fl/fl) (Rbpj-cKO) ureters

Project description:Evaluation of the transcriptional changes in E14.5 ureters upon loss of Rbpj in the ureteric mesenchyme.

2022-01-04 | GSE169661 | GEO

Transcripts identified by microarray analysis that were deregulated in E14.5 Tbx18cre/+;Tbx2fl/fl;Tbx3fl/fl (Tbx2/Tbx3cDKO) ureters

Project description:Evaluation of the transcriptional changes in E14.5 ureters upon combined loss of Tbx2 and Tbx3 in the ureteric mesenchyme.

2018-11-16 | GSE122560 | GEO

Transcripts identified by microarray analysis that were deregulated in E14.5 Tbx18(cre/+);FGFR1(fl/fl);FGFR2(fl/fl) (Fgfr1/2cDKO) ureters

Project description:Evaluation of the transcriptional changes in E14.5 ureters upon loss of Fgfr1 and Fgfr2 in the ureteric mesenchyme.

2022-09-06 | GSE197369 | GEO

15.5dpc Ureteric tree cells Vs rest

Project description:Triplicate pairwise comparsion of FACS sorted GFP+ve Vs GFP-ve cells from the kidneys of the HoxB7-GFP transgenic mice on compugen 22K mouse arrays. HoxB7-GFP mice express GFP in the ureteric tree and its derivatives while the metanephric mesenchyme, interstitium, developing vasculature etc do not. Keywords: repeat sample

2005-07-20 | GSE2281 | GEO

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