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Acinetobacter baumannii Fatty Acid Desaturases Facilitate Survival in Distinct Environments

ABSTRACT: Acinetobacter baumannii Fatty Acid Desaturases Facilitate Survival in Distinct Environments

PROVIDER: PRJNA684913 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13248015_1.fastq.gz	Fastqsanger.gz
	SRR13248015_2.fastq.gz	Fastqsanger.gz
	SRR13248016_1.fastq.gz	Fastqsanger.gz
	SRR13248016_2.fastq.gz	Fastqsanger.gz
	SRR13248017_1.fastq.gz	Fastqsanger.gz

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Acinetobacter baumannii Fatty Acid Desaturases Facilitate Survival in Distinct Environments

Project description:RNA sequencing transcriptomics was performed on a highly multidrug resistant A. baumannii strain belonging to international clone I, AB5075_UW and a transposon insertion inactivated mutant of ABUW_1103 (DesT1), which encodes for a TetR family regulator. Transcriptomics revealed that DesT1 might function as a global regulator controlling expression of various genes involved in fatty acid metabolism and transport and a range of outer membrane proteins. Genes encoding acyl-CoA desaturase and ferredoxin reductase were found to be significantly upregulated when DesT1 regulator was inactivated. Aerobic desaturases are known to play an important role in maintaining membrane homeostasis.

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Expression data from free fatty acid polarized bone marrow Gr1- myeloid cells

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Elevated Dietary Non-Esterified Fatty Acid Levels Impact BeWo Trophoblast Metabolism and Lipid Processing: A Multi-OMICS Outlook

Project description:Maternal obesity and gestational diabetes mellitus (GDM) are associated with adverse intrauterine environments that are high in circulating nutrients. These adverse environments facilitate impairments in placental mitochondrial function and nutrient processing that ultimately leads to an increased risk of the exposed offspring developing non-communicable cardiometabolic health disorders (e.g. obesity, type 2 diabetes, metabolic syndrome). Analysis of fasting serum lipid levels in the third trimester has highlighted that Palmitate (PA) and oleate (OA) are the most abundance non-esterified fatty acids (NEFAs) in circulation. More importantly, these important NEFAs are elevated in both obese and GDM pregnancies. As PA and OA are the most abundant NEFAs in the typical “Westernized Diet” they elevated levels in obese and GDM pregnancies may highlight increased maternal consumption of these fats and could be a link between poor diet and abberant placental function. However, the independent and combined impacts of dietary PA and OA on mitochondrial function and nutrient processing in placental trophoblast cells remains poorly defined. Thus, in this study we sought to utilize a multi-omics approach (combining transcriptomics with untargeted metabolomics and lipidomics) to elucidate the underlying impacts of elevated NEFA levels on BeWo placental trophoblast cells. We highlighted a significant enrichment in the Wikipathways Fatty Acid Biosynthesis Pathway in BeWo cytotrophoblast cells treated with a combination of PA and OA (P/O; 50 µM each). Further, we identified differential expression of genes involved in lipid processing (e.g. ACADVL, ACACB, ACSL5, CREB3L3, PLIN2, and SCD) in BeWo cytotrophoblast cells treated with dietary NEFA species. Overall, these data highlighted that alterations in lipid processing, storage, and oxidation are implicated in the development of placental dysfunction in response to elevated lipid levels.

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Project description:Extreme environments Raw sequence reads

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