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Transcriptome sequencing of cerebrospinal fluid unravels cell landscape and neuroinflammatory signatures in the bacterial meningitis

ABSTRACT: Transcriptome sequencing of cerebrospinal fluid unravels cell landscape and neuroinflammatory signatures in the bacterial meningitis

PROVIDER: PRJNA685349 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13319534_1.fastq.gz	Fastqsanger.gz
	SRR13319534_2.fastq.gz	Fastqsanger.gz
	SRR13319535_1.fastq.gz	Fastqsanger.gz
	SRR13319535_2.fastq.gz	Fastqsanger.gz
	SRR13319536_1.fastq.gz	Fastqsanger.gz

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Project description:Meningitis is a life-threatening condition characterized by the inflammation of the leptomeningeal membranes surrounding the brain and spinal cord. The term meningitis is an umbrella term and includes several different etiologies. The majorities of meningitis cases are caused by viruses (viral meningitis; VM) and are often associated with low mortality rates and low risk of developing neurological. In contrast, meningitis caused by some viral infections, such as tick-borne encephalitis (TBE), can be life-threatening when left untreated with increased risk of developing neurological sequelae. Acute bacterial meningitis (ABM), however, is one of the leading causes of death due to infectious diseases worldwide and is associated with rapid disease progression, high mortality rates and increased risk of long-term neurological sequelae in survivors. As meningitis is caused by numerous different pathogens, the host-response is typically highly variable and it is currently unknown if different pathogens can introduce specific proteome changes in the cerebrospinal fluid (CSF). In this study we applied DIA-MS to provide novel insights for in-depth understanding of central nervous system functioning and host response during meningitis in a cohort of patients with differential diagnosis of meningitis, to account for variability contributed by different disease-causing pathogens. The results reveal drastic changes in the CSF proteome during meningitis, where in particular a massive increase of neutrophil derived proteins in the CSF correlated with ABM, suggesting that activated neutrophils play a particular role in ABM. Additionally both ABM and VM result in marked reduction of brain-specific proteins in the CSF, which could be indicative of pathophysiological mechanisms leading to brain damage. Furthermore, generation of lasso regression model enables separation of ABM with high sensitivity and specificity, demonstrating that several proteins are required to confidently discriminate between ABM, VM and BM.

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Transcriptome sequencing of cerebrospinal fluid unravels cell landscape and neuroinflammatory signatures in the bacterial meningitis

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