Project description:Investigation of whole genome gene expression level changes in early generation Caenorhabditis elegans Bristol N2 prg-1 and Bristol N2 prg-1; daf-2 double mutant, compared to late-generation strains. A seven chip study using total RNA recovered from three separate alleles of Caenorhabditis elegans Bristol N2 prg-1 and four separate allelic combinations of a double mutant strain, Bristol N2 prg-1; daf-2 , in which prg-1 (pk2298) and prg-1 (tm872) are combined with one of three daf-2 alleles (e1368, 1370, m41).
Project description:The determination of molecular pathways that are affected with age and lead to increased disease susceptibility is crucial. The nematode has proven an ideal experimental model to study ageing due to significantly conserved genes governing ageing throughout species. Profiling of gene expression levels in ageing wt Caenorhabditis elegans, utilizing a robust Affymetrix microarray platform, resulted in the identification of age-dependent gene expression alterations that characterize the nematode's ageing process. We used Affymetrix microarrays to detail the global programme of gene expression underlying ageing and identified distinct classes of up- or down-regulated genes during this process.
Project description:Investigation of whole genome gene expression level changes in early generation Caenorhabditis elegans Bristol N2 rsd-2 and Bristol N2 rsd-6 single mutants, compared to late-generation strains at 25°C and 20°C
Project description:Investigation of whole genome gene expression level changes in early generation Caenorhabditis elegans Bristol N2 rsd-2 and Bristol N2 rsd-6 single mutants, compared to late-generation strains at 25°C and 20°C A six chip study using total RNA recovered from two separate alleles of Caenorhabditis elegans Bristol N2 rsd-2 and one allele Bristol N2 rsd-6 , in which the effects of transgeneration aging are studied in early generation animals and late generation animals at the restrictive temperature of 25°C and the permissive temperature, 20°C.