Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Integrated single cell analysis of chromatin accessibility and cell surface markers

ABSTRACT: Integrated single cell analysis of chromatin accessibility and cell surface markers

PROVIDER: PRJNA685560 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Integrated single cell analysis of chromatin accessibility and cell surface markers

Project description:Integrated single cell analysis of chromatin accessibility and cell surface markers

| PRJNA685561 | ENA

Transcription profiling of mouse bipotential embryonic liver cells to identify liver progenitor cell surface markers.

Project description:This SuperSeries is composed of the following subset Series:; GSE6942: Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers (moe430a). GSE6957: Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers (mouse4302). Experiment Overall Design: Refer to individual Series

2008-06-15 | E-GEOD-6966 | biostudies-arrayexpress

Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture

Project description:We differentiated human iPSC to day 14 using our dopaminergic neuron protocol. At day 14, the cells were fixed, stained and FACS sorted in to the FOXA2/LMX1 double positive (wanted neural progenitor population) and double negative pools. We compared the RNA of the pools via microarray to identify surface markers in the double positive pool to be able to live purify the neural progenitors from the rest of the cells. Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can provide sources for midbrain dopaminergic (mDA) neural progenitors (NPCs) for cell therapy to treat Parkinson’s disease (PD) patients. However, the well-known line-to-cell line variability in the differentiation capacity of individual cell lines needs to be improved for the success of this therapy. To address this issue, we sought to identify mDA NPC specific cell surface markers for fluorescence activated cell sorting (FACS). Through RNA isolation after sorting for NPCs based on staining for cell-specific transcription factors followed by microarray, we identified two positive cell surface markers (CORIN and CD166) and one negative cell surface marker (CXCR4) for mDA NPC sorting. These three markers can enrich dopaminergic NPCs to 90% purity, and the sorted NPCs more efficiently differentiate to mature dopaminergic neurons compared to unsorted or CORIN+ alone mDA NPCs. This surface marker identification strategy can be used broadly to facilitate isolation of cell subtypes of interest from heterogeneous cultures. Complete processed data are not available

2017-05-23 | GSE99139 | GEO

Cell surface markers that distinguish human beta cells and alpha cells

Project description:The goal of this experiment is to determine cell surface markers that are present in human beta cells or alpha cells but not both. Cells from human islets were FACS sorted and prepared as matched pairs of beta and alpha cells preparations.

2011-06-10 | E-MTAB-459 | biostudies-arrayexpress

Identifying novel surface markers for megakaryocyte progenitor cells [D27 transgene]

Project description:We carried out 10X sequencing of D27 induced pluripotent stem cell-derived megakaryocyte population which had been sorted for surface markers of megakaryocyte progenitors.

2022-02-23 | GSE168289 | GEO

Identifying novel surface markers for megakaryocyte progenitor cells [10x D27]

Project description:We carried out 10X sequencing of D27 induced pluripotent stem cell-derived megakaryocyte population which had been sorted for surface markers of megakaryocyte progenitors.

2022-02-23 | GSE166726 | GEO

Heterogeneous expression of cell surface markers in human myeloma cell lines: implications for disease modelling and therapeutic targeting

Project description:This RNAseq analysis is performed as a part of the work "Heterogeneous expression of cell surface markers in human myeloma cell lines: implications for disease modelling and therapeutic targeting", where expression levels both at the cell surface level and transcriptomic level are analysed.

2024-10-03 | GSE250236 | GEO

Identifying novel surface markers for megakaryocyte progenitor cells

Project description:Identifying novel surface markers for megakaryocyte progenitor cells

| PRJNA706752 | ENA

Integrated single-cell multiomics reveals novel immune candidate markers for post-traumatic coagulopathy

Project description:Integrated single-cell multiomics reveals novel immune candidate markers for post-traumatic coagulopathy

| PRJNA925472 | ENA

Identification of cell surface markers and establishment of monolayer differentiation protocol for retinal pigment epithelial cells.

Project description:In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells for cell based reparative therapy of age-related macular degeneration (AMD). In the present study, we identify CD140b, CD56, GD2 and CD184 as central cell surface markers to evaluate hPSC-RPE differentiation efficiency, as well as a potential tool for the enrichment of hPSC-RPE during and after differentiation. Using these markers together with single cell RNA sequencing to evaluate the differentiation process, we have established an efficient xeno-free and defined monolayer differentiation methodology where culture on supportive human recombinant laminin eliminates the need for manual selection, allowing large-scale production of pure hPSC-RPE.

2020-02-29 | E-MTAB-7742 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data