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Genome-wide binding of transcription factor IRF3

ABSTRACT: Genome-wide binding of transcription factor IRF3

PROVIDER: PRJNA686372 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13272072_1.fastq.gz	Fastqsanger.gz
	SRR13272072_2.fastq.gz	Fastqsanger.gz
	SRR13272073_1.fastq.gz	Fastqsanger.gz
	SRR13272073_2.fastq.gz	Fastqsanger.gz
	SRR13272074_1.fastq.gz	Fastqsanger.gz

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Genome-wide binding of transcription factor IRF3

Project description:Genome-wide binding of IRF3 was analysed in MM1.S cells using ChIP-seq high-throughput approach. MM1.S cells were fixed in 1% formaldehyde (Alfa Aesar) for 15min at room temperature. cells were lysed with hypotonic lysis buffer for 15minutes followed by nuclear lysis buffer for 15min on ice. ChIP for IRF3 performed by a standard ChIP-seq protocol and the genome-wide binding of IRF3 data was analysed which revealed peaks enrichment at cell cycle genes TSS.

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Project description:This SuperSeries is composed of the SubSeries listed below. In vivo transcription factor binding is regulated by DNA sequence and chromatin features. However, the impact of chromatin context on genome-wide transcription factor binding affinities have not yet been characterized. Here we report the establishment of a quantitative protein-DNA binding assay to determine genome-wide binding affinities to native chromatinized DNA. We use this method to quantify apparent genome-wide binding affinities of both pioneering and non-pioneering transcription factors to uncover the regulatory role of chromatin on transcription factor binding. This revealed that DNA accessibility is the main determinant of transcription factor binding, which restricts nanomolar binding of non-pioneering factors to promoters. DNA binding motifs only appear to be important for very high-affinity binding, but are not essential for nanomolar binding. We uncover numerous biological processes enriched at specific binding affinities, suggesting they are regulated at defined transcription factor concentrations. Importantly, our method adds a new quantitative layer of transcription factor biology, enabling stratification of genomic targets based on transcription factor expression and prediction of transcription factor binding sites under non-physiological conditions, such as disease associated elevated expression of (onco)genes.

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