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Ruminant gut microbes

ABSTRACT: Ruminant gut microbes Raw sequence reads

PROVIDER: PRJNA688363 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR13345700_1.fastq.gz	Fastqsanger.gz
	SRR13345700_2.fastq.gz	Fastqsanger.gz
	SRR13345701_1.fastq.gz	Fastqsanger.gz
	SRR13345701_2.fastq.gz	Fastqsanger.gz
	SRR13345702_1.fastq.gz	Fastqsanger.gz

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Next Generation RNA Sequencing - Delineation of Hepatic Transcriptome Governed by Liver Clock and Gut Microbes over Time

Project description:We report the application of bulk RNA-sequencing-based technology for high-throughput profiling to examine the individual and combinatorial effects of the liver circadian clock and gut microbes on the liver transcriptome over 24-hours. Principle Component Analysis demonstrated that functionality of the liver circadian clock is the primary driver of the hepatic transcriptome profile, and presence of microbes is the secondary driver. We identified a range of significantly oscillating transcripts within each experimental group using empirical_JTK_CYCLE, and revealed an overall increase in oscillating transcripts with both the loss of cuntional liver clock and gut microbes. Network analysis via Spearman correlation revealed that a broken liver clock results in increased connections and correlated transcripts only in the presence of gut microbes. Finally, we show by differential expression and gene set enrichment analysis that several key metabolic pathways, particularly carbohydrate and lipid metabolism, were significantly downregulated when the liver clock is broken, regardless of microbial status. This study demonstrates the complex contributions of the liver circadian clock and gut microbes in transcriptome programming, both over time and overall.

2022-06-06 | GSE184303 | GEO

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