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Comparative transcriptomic analysis of in the hindbrain of olive flounder (Paralichthys olivaceus) with distinct personality and oxygen metabolism

ABSTRACT: Comparative transcriptomic analysis of in the hindbrain of olive flounder (Paralichthys olivaceus) with distinct personality and oxygen metabolism

PROVIDER: PRJNA689319 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13344952_1.fastq.gz	Fastqsanger.gz
	SRR13344952_2.fastq.gz	Fastqsanger.gz
	SRR13344953_1.fastq.gz	Fastqsanger.gz
	SRR13344953_2.fastq.gz	Fastqsanger.gz
	SRR13344954_1.fastq.gz	Fastqsanger.gz

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Comparative transcriptomic analysis of in the hindbrain of olive flounder (Paralichthys olivaceus) with distinct personality and oxygen metabolism

Project description:In previous studies, we employed multiple behavior assays, including propensity to feed, simulated trawl capture and escape response, to prove the presence of bold and shy personality in olive flounder. However, the molecular mechanism of the different personality has not been elucidated. In the present study, the transcriptomic profiles of the hindbrain from flounder with distinct personalities were compared. A total of 144 differently expressed genes were identified, including 74 up-regulated and 70 downregulated genes. Genes involved in hypoxia stress were detected in SP flounder, accompanied by down-regulation of ribosomal RNA synthesis. In addition, genes related to calcium signaling pathway, including endothelin, b-Fos, c-Fos and c-Jun were up-regulated in SP flounder. Furthermore, personality-related genes, including UI, CCK, c-Fos showed a significantly higher level in SP flounder compared with BP flounder. GO enrichment analysis indicated that the GO categories “the tight junction pathway” and “lipid transport or localization pathway” are enriched in SP flounder, suggesting that the central nervous system homeostasis would be compromised. Finally, a simple and scalable DNA methylation profiling allows for methylation analysis for different genes. The results found that part of gene expression is negatively related to methylation of promoter. Altogether, identification of the related genes in flounder with different personalities will shed new light to improve critical industry issues related to stress and increase aquaculture production of flounder.

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