Project description:Lysosomes are cellular recycling stations and metabolic signaling hubs. Whether lysosome dynamics regulate mammalian brain development is unknown. We found that radial glia cells possess a large number of endolysosomes and that asymmetric inheritance of lysosomes in daughters of radial glia cells can predict fate and cell cycle length. To determine the lysosomal regulation of translation initiation by mTORC1/eIF4E axis, we performed RNA immunoprecipitation sequencing (RIP-seq) with antibody against eIF4E in E13.5 neocortex.
Project description:Lysosomes are cellular recycling stations and metabolic signaling hubs. Whether lysosome dynamics regulate mammalian brain development is unknown. We found that radial glia cells possess a large number of endolysosomes and that asymmetric inheritance of lysosomes in daughters of radial glia cells can predict fate and cell cycle length. To determine the translation control by the mTORC1, we performed polysome profiling of mRNAs associated with >3 ribosomes for neural progenitor cells that treated with DMSO or Torin1 for 2 hours.
Project description:Grain samples of Hordeum vulgare L. sensu lato variety Sebastian were obtained from plants grown in the fields at four breeding stations in 2017, representing seed development under different combinations of temperature and rainfall.Sample description:CAS [1-4] - location n. 5 - (Caslav) - high yield, above average temperature and high rainfall.UHO [1-4] - location n. 11 (Uhersky Ostroh) - low yield, high temperature and low rainfall.CHA [1-4] - location n. 2 (Chrastava) - average yield, low temperature, very high rainfall.KRU [1-4] - location n. 8 (Krasne Udoli) - average yield, low temperature, average rainfall.
Project description:Patients with chronic rhinosinusitis (CRS) have abnormal immune responses triggered by a variety of infectious agents, airborne toxins and fungi. Respiratory epithelial cells serve as relay stations capable of amplifying or augmenting cues received from external stimuli to nearby immune cells located in the sinus mucosa. Previous studies have identified increases in complement components and complement gene expression in the mucosa of patients with atopic CRS with nasal polyps (CRSwNP). As part of a larger study, we used shotgun proteomics to quantify changes in mucus proteins between patients with and without nasal polyps.