Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of the most toxic 6mer seed consensus and some of its targets in human cells

ABSTRACT: Identification of the most toxic 6mer seed consensus and some of its targets in human cells

PROVIDER: PRJNA689807 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13361401_1.fastq.gz	Fastqsanger.gz
	SRR13361401_2.fastq.gz	Fastqsanger.gz
	SRR13361402_1.fastq.gz	Fastqsanger.gz
	SRR13361402_2.fastq.gz	Fastqsanger.gz
	SRR13361403_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Identification of the toxic 6mer seed consensus for human cancer cells

Project description:6mer seed toxicity is a novel cell death mechanism that kills cancer cells by triggering death induced by survival gene elimination (DISE). It is based on si- or shRNAs with a specific G-rich nucleotide composition in position 2-7 of their guide strand. An arrayed screen of 4096 6mer seeds on two human and two mouse cell lines identified a consensus GGGGGC as the most toxic seed. We have now tested two additional cell lines, one human and one mouse, confirming the GGGGGC consensus as the most toxic average 6mer seed for human cells while slightly less significant for mouse cells. RNA Seq and bioinformatics analyses suggested that an siRNA containing the GGGGGC seed (siGGGGGC) is toxic to cancer cells by targeting GCCCCC seed matches in the 3' UTR of a set of genes critical for cell survival. We have identified several genes targeted by this seed and demonstrate direct and specific targeting of GCCCCC seed matches, which is attenuated upon mutation of the GCCCCC seed matches in these 3' UTRs. Our data show that siGGGGGC kills cancer cells through its miRNA-like activity and points at artificial miRNAs, si- or shRNAs containing this seed as a potential new cancer therapeutics

2022-02-16 | GSE196839 | GEO

Identification of the most toxic 6mer seed consensus and some of its targets in human cells

Project description:We provide evidence that the 6mer seed GGGGGC is toxic in human cells by targeting GCCCCC seed matches in the 3' UTR oif a set of genes critical for cell survival thereby inducing DISE

2021-12-31 | GSE164289 | GEO

Identification of the toxic 6mer seed consensus for human cancer cells

Project description:Identification of the toxic 6mer seed consensus for human cancer cells

| PRJNA807523 | ENA

6mer seed toxicity in tumour suppressive microRNAs

Project description:6mer seed toxicity in tumour suppressive microRNAs

2018-09-11 | GSE111379 | GEO

6mer seed toxicity in viral microRNAs

Project description:We provide evidence that viral miRNAs use 6mer seed toxicity to kill cells

2019-11-13 | GSE135453 | GEO

SPOROS: A pipeline to analyze DISE/6mer Seed Toxicity

Project description:The SPOROS pipeline is a read-based sequence analysis pipeline for studying 6mer Seed Toxicity in mouse and human RNA-seq data.

2021-08-22 | GSE182222 | GEO