Project description:In this study, we performed an RNA-Seq transcriptomic analysis concerning acetic acid bacteria’s acid resistance mechanisms during a continuous and periodical industrial submerged vinegar fermentation process, where the acetic acid concentration fluctuates between ~8% and ~12%
Project description:Populations of engineered metabolite-producing microorganisms are prone to evolutionary production declines during industrial-scale cultivations. In this study, we develop a synthetic product addiction system in E coli that addicts mevalonic acid production cells to mevalonic acid. Through experimentally simuluated long-term fermentation, we investigate how product-addicted organisms remain stable and avoid formation of genetic subpopulations of fit, non-producing cells.