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Distinct B cell repertoires characterize patients with mild and severe COVID-19 [GEX]

ABSTRACT: Distinct B cell repertoires characterize patients with mild and severe COVID-19 [GEX]

PROVIDER: PRJNA690255 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13376428_1.fastq.gz	Fastqsanger.gz
	SRR13376428_2.fastq.gz	Fastqsanger.gz
	SRR13376429_1.fastq.gz	Fastqsanger.gz
	SRR13376429_2.fastq.gz	Fastqsanger.gz
	SRR13376430_1.fastq.gz	Fastqsanger.gz

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Distinct B cell repertoires characterize patients with mild and severe COVID-19 [GEX]

Project description:Protective immunity against COVID-19 likely depends on the production of SARS-CoV-2 binding plasma cells and memory B cells after infection or vaccination. Previous work has shown evidence that germinal center reactions, a critical component of the B cell response, are disrupted in severe COVID-19. This may adversely affect protective immunity from re-infection. Consistent with an extrafollicular B cell response, severe COVID-19 patients have large scale changes in B cell populations such as elevated frequencies of clonally expanded, class switched, unmutated plasmablasts. However, it is not clear whether mild or asymptomatically infected individuals show similar differences in B cell repertoires. Here, we use single cell RNA sequencing of B cells to show that, in contrast to hospitalized COVID-19 patients, mildly symptomatic COVID-19 subjects have B cell repertoires skewed towards clonally diverse, somatically hypermutated memory B cells approximately 30 days after the onset of symptoms. This provides evidence that B cell responses are less disrupted in mild COVID-19, and that the infection resolves with the production of memory B cells.

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Distinct B cell repertoires characterize patients with mild and severe COVID-19

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