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Global gene regulation by RNA-binding protein NrdA in Aspergillus luchuensis mut. kawachii

ABSTRACT: Global gene regulation by RNA-binding protein NrdA in Aspergillus luchuensis mut. kawachii

PROVIDER: PRJNA690526 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13377181_1.fastq.gz	Fastqsanger.gz
	SRR13377181_2.fastq.gz	Fastqsanger.gz
	SRR13377182_1.fastq.gz	Fastqsanger.gz
	SRR13377182_2.fastq.gz	Fastqsanger.gz
	SRR13377183_1.fastq.gz	Fastqsanger.gz

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Global gene regulation by RNA-binding protein NrdA in Aspergillus luchuensis mut. kawachii

Project description:We report that the RNA-binding protein NrdA has a significant role on the global mRNA expression in the white koji fungus, Aspergillus luchuensis mut. kawachii. We constructed the nrdA conditional expression strain using the Tet-On system in A. kawachii. Downregulation of nrdA caused severe growth defect, indicating that NrdA is essential for proliferation of A. kawachii. Parallel RNA-seq and RNA immunoprecipitation (RIP)-seq analysis identified potential NrdA-interacted transcripts, corresponding to 32% of predicted protein coding genes of A. kawachii. Subsequent gene ontology analysis suggested that overexpression of NrdA affects a variety of metabolic activity including secondary metabolite production.

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Characterization of epigenetic regulators, HepA and LaeA, in Aspergillus luchuensis mut. kawachii

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