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Bulk RNA sequencing of WT and Pax6-KO mouse lacrimal gland organoids under expansion and differentiation conditions

ABSTRACT: Bulk RNA sequencing of WT and Pax6-KO mouse lacrimal gland organoids under expansion and differentiation conditions

PROVIDER: PRJNA690793 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13388600_1.fastq.gz	Fastqsanger.gz
	SRR13388600_2.fastq.gz	Fastqsanger.gz
	SRR13388601_1.fastq.gz	Fastqsanger.gz
	SRR13388601_2.fastq.gz	Fastqsanger.gz
	SRR13388602_1.fastq.gz	Fastqsanger.gz

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Bulk RNA sequencing of WT and Pax6-KO mouse lacrimal gland organoids under expansion and differentiation conditions

Project description:First, we determined the gene expression profile of WT mouse lacrimal gland tissue and organoids in expansion and in differentiation. Then, we sought the differences between WT and Pax6-KO mouse lacrimal gland organoids in expansion and in differentiation.

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Project description:Background: The study of human lacrimal gland biology and development is limited. Lacrimal gland tissue is damaged or poorly functional in a number of disease states including dry eye disease. Development of cell based therapies for lacrimal gland diseases requires a better understanding of the gene expression and signaling pathways in lacrimal gland. Differential gene expression analysis between lacrimal gland and other embryologically similar tissues may be helpful in furthering our understanding of lacrimal gland development. Methods: We performed global gene expression analysis of human lacrimal gland tissue using Affymetrix ® gene expression arrays. Primary data from our laboratory was compared with datasets available in the NLM GEO database for other surface ectodermal tissues including salivary gland, skin, conjunctiva and corneal epithelium. Results: The analysis revealed statistically significant difference in the gene expression of lacrimal gland tissue compared to other ectodermal tissues. The lacrimal gland specific, cell surface secretory protein encoding genes and critical signaling pathways which distinguish lacrimal gland from other ectodermal tissues are described. Conclusions: Differential gene expression in human lacrimal gland compared with other ectodermal tissue types revealed interesting patterns which may serve as the basis for future studies in directed differentiation among other areas.

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Project description:RNA-Seq of embryonic, adult lacrimal gland and lacrimal gland stem cells

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