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Bulk segregant RNA seq samples from young leaf tissue of sesame seedlings with either SiCD-A or SiCD-B allele

ABSTRACT: Bulk segregant RNA seq samples from young leaf tissue of sesame seedlings with either SiCD-A or SiCD-B allele

PROVIDER: PRJNA691037 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13397574_1.fastq.gz	Fastqsanger.gz
	SRR13397574_2.fastq.gz	Fastqsanger.gz
	SRR13397575_1.fastq.gz	Fastqsanger.gz
	SRR13397575_2.fastq.gz	Fastqsanger.gz
	SRR13397576_1.fastq.gz	Fastqsanger.gz

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Bulk segregant RNA seq samples from young leaf tissue of sesame seedlings with either SiCD-A or SiCD-B allele

Project description:Transcriptome analysis was performed on young leaves from 17-day old sesame seedling. The experiment aimed to shed light on the polar regulatory gene network in sesame under KANADI1 loss of function and identify candidate genes underlie the hyponastic leaf phenotype. We compaired between homozygous lines for either SiCD-A allele contains KAN1 (WT) or SiCD-B allele contains kan1 (mutant) on S.indicum LG8.

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Project description:Sesame seeds is an important traditional crop with high oil content and other abundant nutrients which are very beneficial for diet and health of human being. However, the molecular mechanism for metabolite accumulation, especially for oil and phenylpropanoid biosynthesis, is still not very clear in sesame. In this study, the transcriptome profiles of black and white sesame seeds were compared by RNA-sequencing. Transcriptome analysis showed that the expression patterns of genes encoding phenylpropanoid pathway enzymes were different between the two sesame cultivars. Compared with white sesame, most of genes involved in oil biosynthesis were significantly down-regulated in black sesame.

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Serine and SAM responsive complex SESAME regulates histone modification crosstalk by sensing cellular metabolism

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Serine and SAM responsive complex SESAME regulates histone modification crosstalk by sensing cellular metabolism

Project description:We report the genome-wide localization of SESAME and found that three SESAME subunits localized in the gene body. Examining the genome-wide localization of SESAME subunits (Sam1, Ser33, Pyk1) and Set1

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Project description:The purpose is to analyze the effect of SESAME complex on gene transcription

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Project description:The purpose is to analyze the effect of SESAME complex on gene transcription

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Bulk segregant sequencing (Bulk-Seq) analysis to identify paternal suppressors of medea in Arabidopsis thaliana

Project description:Arabidopsis thaliana seeds that maternally inherit a medea (mea) mutant allele abort before completing embryogenesis. However, mea seeds can be rescued by pollen from several natural ecotypes of A. thaliana, including the Cape Verdian accession Cvi-0. We developed a method for the mapping of parent-of-origin effects using whole-genome sequencing of segregant bulks. The strategy is to create an F2 population that contains one set of chromosomes from the maternal parent (mea, in a Ler background) but inherits two segregating sets (Ler and Cvi-0) from the other parent. The two paternally segregating sets have opposite effects in mea penetrance: Ler fathers allow full mea seed abortion, while Cvi fathers rescue 90% of medea seeds. Therefore, the two segregating paternal sets are not equally transmitted to the next generation. DNA extracted from pools of viable F2 seedlings was sequenced on an Illumina HiSeq 2000 platform, mapped to the reference TAIR10 A. thaliana genome and the ratio between Ler and Cvi-0 SNPs used to identify chromosomal regions enriched in Cvi-0 sequences. As a control, DNA pools extracted from crosses between a wild-type Ler mother and a hybrid Cvi-0:Ler father were also sequenced. Three biological replicates were made for each pool. Ler-1 x Ler-1:Cvi-0 hybrid viable seedlings pools (each is a biological replicate): WT_pool_1, WT_pool_2, WT_pool_3 mea-1/mea-1; MEA-GR x Ler-1:Cvi-0 hybrid viable seedlings pool (each is a biological replicate): mea_pool_1, mea_pool_2, mea_pool_3

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