Project description:Berberin has been shown to inhibit 3T3L1 preadipocytes differentiation. This study aims to determine the genes regulated by berberine and clarify the possible molecular mechanism underlying the action.
Project description:3T3L1 preadipocytes were treated with DMSO control and Nutlin-3a (prepared in DMSO) and total RNA extracted from the treated cells were subjected to illumina based RNA-seq to analyze and compare transcriptome to understand gene expression regulation influenced by p53 activation via Nutlin-3a treatment.
Project description:Berberine shows protective effect against AAPH-damaged C17.2 neural stem cells. This study aims to determine the genes regulated by berberine and clarify the possible molecular mechanism underlying the action.
Project description:Berberin has been shown to inhibit HepG2 cell viability. This study aims to determine the genes regulated by berberine and clarify the possible molecular mechanism underlying the action.
Project description:Maps of genomic regions in proximity to the nuclear lamina were determined in undifferentiated 3T3L1 preadipocytes and 9 day differentiated (wild type, and under Tmem120a or Tmem120a/b knockdown) adipocytes using DamID with a Dam-Lamin B1-encoding lentivirus.
Project description:We profiled PPARg dependent gene expression changes during differntiation of 3T3L1 cell using PPARg siRNA 3T3-L1 (Pre-adipocyte) cell line was induced to differentiate using standard adipocyte differentiation media (IBMX, Dex and Insulin) 48hrs post-confluency. RNA was harvested at day -2 (confluent fibroblasts), 48hrs post-induction with IBMX, DEX and Insulin (day=0) and for each subsequent day after rosiglitazone treatment. Illumina beadchip microarrays were used to determine expression profiles of genes differentially regulated in cells transfected with either siRNA targeting PPARgamma or a non-targeting control siRNA. 3T3L1 cell were induced to differentiate into adipocytes using IBMX, DEX and Insulin. RNA from cell treated with PPARg-specific siRNA and non-specific siRNA was isolated at different timepoints. Illumina MouseRef-8 v1.1 Bead chips were used for expression profiling
Project description:Identifying changes in gene expression in 3T3L1 cell line treated with or without insulin. "Microbiota and adipocyte mitochondrial damage in type 2 diabetes are linked by Mmp12+ macrophages"