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Single cell RNA-seq reveals the urothelium and stroma cell heterogeneity in acute and chronic injured urinary bladder by single-cell analysis

ABSTRACT: Single cell RNA-seq reveals the urothelium and stroma cell heterogeneity in acute and chronic injured urinary bladder by single-cell analysis

PROVIDER: PRJNA691212 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13402376_1.fastq.gz	Fastqsanger.gz
	SRR13402376_2.fastq.gz	Fastqsanger.gz
	SRR13402377_1.fastq.gz	Fastqsanger.gz
	SRR13402377_2.fastq.gz	Fastqsanger.gz
	SRR13402378_1.fastq.gz	Fastqsanger.gz

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Single cell RNA-seq reveals the urothelium and stroma cell heterogeneity in acute and chronic injured urinary bladder by single-cell analysis

Project description:Some studies have demonstrated the heterogeneity of bladder epithelium and fibroblasts, as well as their crosstalk during bladder development and regeneration. However, due to the limitations of techniques, a comprehensive understanding of cellular heterogeneity and crosstalk during bladder regeneration is still lacking. Here, we used droplet-based scRNA-seq to recover the heterogeneity of urothelial cells, cell cycle characteristic of proliferative cell population and proliferative niches after acute and chronic injury induced by cyclophosphamide.

2021-05-12 | GSE164557 | GEO

Single cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics.

Project description:Objectives: Much of the information to date in terms of subtypes and function of bladder urothelial cells were derived from anatomical location or by the expression of a small number of marker genes. To have a comprehensive map of the cellular anatomy of bladder urothelial cells, we performed single-cell RNA-sequencing to thoroughly characterize mouse bladder urothelium. Materials and methods: A total of 18,917 single cells from mouse bladder urothelium was analyzed by unbiased single-cell RNA sequencing. The expression of the novel cell marker was confirmed by immunofluorescence using urinary tract infections models. Results: Unsupervised clustering analysis identified 8 transcriptionally distinct cell subpopulations from mouse bladder urothelial cells. We discovered a novel type of bladder urothelial cells marked by Plxna4 that may be involved with host response and wound healing. We also found a group of basal-like cells labeled by ASPM that could be the progenitor cells of adult bladder urothelium. ASPM+ urothelial cells are significantly increased after injury by UPEC. In addition, specific transcription factors were found to be associated with urothelial cell differentiation. At the last, a number of interstitial cystitis/bladder pain syndrome-regulating genes were found differentially expressed among different urothelial cell subpopulations. Conclusions: Our study provides a comprehensive characterization of bladder urothelial cells, which is fundamental to understanding the biology of bladder urothelium and associated bladder disease.

2020-12-12 | GSE163029 | GEO

Single Cell Atlas of Injured Sciatic Nerve Tissue

Project description:Single Cell Atlas of Injured Sciatic Nerve Tissue

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Single cell RNA-Seq of Proliferative (NMU-O-P) and Differentiated (NMU-O-D) Normal Mouse Urothelium Organoids

Project description:Single cell RNA-seq was performed in Proliferative (n = 6826) and Differentiated (n = 4896) Normal Mouse Urothelium Organoids to get insight into the differentiation program activated in both conditions.

2019-08-06 | GSE131909 | GEO

Injured kidney organoids gene expression profile at single cell

Project description:Injured kidney organoids gene expression profile at single cell

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Heme metabolism confronts urinary pathogens at the urothelium

Project description:Heme metabolism confronts urinary pathogens at the urothelium

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Single cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics.

Project description:Single cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics.

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Single-cell RNA-sequencing of bleomycin-injured young and old mice

Project description:Single-cell RNA-sequencing of bleomycin-injured young and old mice

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Single Cell Atlas of Injured Sciatic Nerve Tissue

Project description:Sciatic nerve crush (SNC) triggers sterile inflammation within the distal nerve and de-afferented dorsal root ganglia (DRGs). In the nerve, neutrophils and pro-inflammatory Ly6Chigh monocytes appear first and rapidly give way to Ly6Clow resolving macrophages. Transcriptional profiling of injured nerve tissue identifies six macrophage subpopulations, repair Schwann cells and mesenchymal cells as the main cell types. Macrophages at the nerve crush site are distinct from macrophages associated with degenerating nerve fibers. Monocytes and macrophages in the injured nerve “eat” apoptotic cell corpses of leukocytes and thereby contribute to an anti-inflammatory milieu. Studies with chimeric mice show that following SNC few blood-derived immune cells enter DRGs. Myeloid cells in the injured nerve, but not DRGs, express the receptor for the chemokine GM-CSF. In the absence of GM-CSF, conditioning-lesion induced regeneration of DRG neuron central projections is abrogated. Thus, a carefully orchestrated immune response in the nerve is required for conditioning-lesion induced neurorepair.

2021-03-17 | GSE153762 | GEO

Interleukin-17 governs hypoxic adaptation of injured epithelium [CITE-seq & single-cell RNA-seq]

Project description:To define the composition of “first responder” lymphocytes that may engage in crosstalk with injured epithelium we profiled the lymphoytes from unwounded health skin and skin from different time points after wounding with CITE-seq and single-cell RNA-sequencing.

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