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Direct contribution of skeletal muscle mesenchymal progenitors to bone repair

ABSTRACT: Direct contribution of skeletal muscle mesenchymal progenitors to bone repair

PROVIDER: PRJNA691234 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13402851_1.fastq.gz	Fastqsanger.gz
	SRR13402851_2.fastq.gz	Fastqsanger.gz
	SRR13402852_1.fastq.gz	Fastqsanger.gz
	SRR13402852_2.fastq.gz	Fastqsanger.gz
	SRR13402853_1.fastq.gz	Fastqsanger.gz

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Direct contribution of skeletal muscle mesenchymal progenitors to bone repair

Project description:Bone regeneration involves skeletal stem/progenitor cells within periosteum and bone marrow, the formation of a fibrous callus followed by the deposition of cartilage and bone matrix to consolidate the fracture. Interactions between bone and skeletal muscle are known to play a role in bone repair but the underlying mechanisms are poorly understood. To better understand the role of skeletal muscle during bone repair, we characterized stem/progenitor cells within skeletal muscle that participate in bone repair. We show that cells originating from bone marrow, periosteum and skeletal muscle are all derived from the Prx1 embryonic lineage. We developed a mouse polytrauma model combining a non-stabilized tibial fracture and mechanical injury to adjacent skeletal muscles. In this polytrauma model, bone fracture healing is impaired. We characterized the Prx1-derived cell population within skeletal muscle in response to fracture and to polytrauma. To do so, we performed fracture and polytrauma in Prx1Cre;Rosa mTmG mice. We harvested skeletal muscle surrounding the tibia at d0 (uninjured), and surrounding the fracture site at d3 and d5 post-fracture or post-polytrauma. Following enzymatic and mechanical digestion of skeletal muscle tissue, we FACS sorted Prx1-derived GFP+ cells and sequenced them using the 10X Chromium technology.

2021-03-04 | GSE164573 | GEO

Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.

Project description:Bone regenerates by activation of tissue resident stem/progenitor cells, formation of a fibrous callus followed by deposition of cartilage and bone matrices. Here, we show that mesenchymal progenitors residing in skeletal muscle adjacent to bone mediate the initial fibrotic response to bone injury and also participate in cartilage and bone formation. Combined lineage and single-cell RNA sequencing analyses reveal that skeletal muscle mesenchymal progenitors adopt a fibrogenic fate before they engage in chondrogenesis after fracture. In polytrauma, where bone and skeletal muscle are injured, skeletal muscle mesenchymal progenitors exhibit altered fibrogenesis and chondrogenesis. This leads to impaired bone healing, which is due to accumulation of fibrotic tissue originating from skeletal muscle and can be corrected by the anti-fibrotic agent Imatinib. These results elucidate the central role of skeletal muscle in bone regeneration and provide evidence that skeletal muscle can be targeted to prevent persistent callus fibrosis and improve bone healing after musculoskeletal trauma.

| S-EPMC8128920 | biostudies-literature

Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

Project description:Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

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Fibroblasts direct differentiation of human breast epithelial progenitors

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Neonatal Skeletal Progenitors

Project description:Neonatal Skeletal Progenitors

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Overloading mesenchymal progenitors (FAPs)

Project description:Overloading mesenchymal progenitors (FAPs)

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Overloading mesenchymal progenitors (FAPs)

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Localized EMT reprograms glial progenitors to promote spinal cord repair

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Direct Conversion of Human Fibroblasts into Neuronal Restricted Progenitors

Project description:Neuronal restricted progenitors (NRPs) represent a type of transitional intermediate cells that lie between multipotent neural progenitors (NPs) and terminal differentiated neurons during neurogenesis. These NRPs have the ability to self-renew and differentiate into neurons, but not into glial cells, which is considered as an advantage for cellular therapy of human neurodegenerative diseases. However, difficulty in the extraction of highly purified NPRs from normal nervous tissue prevents further studies and applications. In this study, we reported conversion of human fetal dermal fibroblasts into human induced neuronal restricted progenitors (hiNRPs) in seven days by using just three defined factors: Sox2, c-Myc, and either Brn2 or Brn4. The hiNRPs exhibited distinct neuronal characteristics, including cell morphology, multiple neuronal markers expression, self-renewal capacity, and genome-wide transcriptional profile. Moreover, hiNRPs were able to differentiate into various terminal neurons with functional membrane properties, but not glial cells. Direct generation of hiNRPs from somatic cells will provide a new source of cells for cellular replacement therapy of human neurodegenerative diseases. This is a general expression microarray design (NimbleGen platform). It includes 5 samples.

2013-10-24 | E-GEOD-51580 | biostudies-arrayexpress

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