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Effect of N1-acetylspermidine treatment on the translatome of hair follicle stem cell organoid cultures

ABSTRACT: Effect of N1-acetylspermidine treatment on the translatome of hair follicle stem cell organoid cultures

PROVIDER: PRJNA692108 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13435518.fastq.gz	Fastqsanger.gz
	SRR13435519.fastq.gz	Fastqsanger.gz
	SRR13435520.fastq.gz	Fastqsanger.gz
	SRR13435521.fastq.gz	Fastqsanger.gz
	SRR13435522.fastq.gz	Fastqsanger.gz

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Effect of N1-acetylspermidine treatment on the translatome of hair follicle stem cell organoid cultures

Project description:Stem cell differentiation has been shown to involve an increase in mRNA translation. Amongst others, the rate of translation is influenced by availability of the natural polyamines putrescine, spermidine, and spermine that are essential for cell growth and modulate stem cell maintenance. However, the link between polyamines and translation in cell fate decisions remains obscure. Here, we used hair follicle stem cell (HFSC) organoids to study the role of polyamines as key regulators of mRNA translation in stem cell fate decisions. HFSCs showed lower translation and reduced polyamine levels than progenitor cells. Surprisingly, we found that reduced translation achieved by changed polyamine availability and stemness do not correlate in the organoids. We identified N1-acetylspermidine as regulator of cell fate decisions. To investigate the effect on translation upon N1-AcSpd supplementation, we performed ribosome foot pronting. We confirmed the increase in proliferation identified by 3'RNA-sequencing on the translatome level. N1-AcSpd treatment partially aligns the translatome of progenitor cells to that of HFSCs. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions.

2021-04-23 | GSE164806 | GEO

Effect of N1-acetylspermidine treatment on hair follicle stem cell organoid cultures

Project description:Stem cell differentiation has been shown to involve an increase in mRNA translation. Amongst others, the rate of translation is influenced by availability of the natural polyamines putrescine, spermidine, and spermine that are essential for cell growth and modulate stem cell maintenance. However, the link between polyamines and translation in cell fate decisions remains obscure. Here, we used hair follicle stem cell (HFSC) organoids to study the role of polyamines as key regulators of mRNA translation in stem cell fate decisions. HFSCs showed lower translation than progenitor cells, and a forced suppression of translation by direct targeting of the ribosome or through specific depletion of natural polyamines elevated stemness. In addition, we identified N1-acetylspermidine as a novel parallel regulator of cell fate decisions. To identify the molecular mechanism by which N1-AcSpd supplementation promotes stemness, we performed RNA-sequencing. We unravel an increase in proliferation. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions.

2021-04-27 | GSE149704 | GEO

Effect of N1-acetylspermidine treatment on hair follicle stem cell organoid cultures

Project description:Effect of N1-acetylspermidine treatment on hair follicle stem cell organoid cultures

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Less invasive diagnostics for cancer using hair follicle

Project description:Primary outcome(s): Comparison of the gene expression profile in hair follicle between cancer patients and healthy volunteer

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N1-acetylspermidine is a determinant of hair follicle stem cell fate.

Project description:Stem cell differentiation is accompanied by increased mRNA translation. The rate of protein biosynthesis is influenced by the polyamines putrescine, spermidine and spermine, which are essential for cell growth and stem cell maintenance. However, the role of polyamines as endogenous effectors of stem cell fate and whether they act through translational control remains obscure. Here, we investigate the function of polyamines in stem cell fate decisions using hair follicle stem cell (HFSC) organoids. Compared to progenitor cells, HFSCs showed lower translation rates, correlating with reduced polyamine levels. Surprisingly, overall polyamine depletion decreased translation but did not affect cell fate. In contrast, specific depletion of natural polyamines mediated by spermidine/spermine N1-acetyltransferase (SSAT; also known as SAT1) activation did not reduce translation but enhanced stemness. These results suggest a translation-independent role of polyamines in cell fate regulation. Indeed, we identified N1-acetylspermidine as a determinant of cell fate that acted through increasing self-renewal, and observed elevated N1-acetylspermidine levels upon depilation-mediated HFSC proliferation and differentiation in vivo. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions. This article has an associated First Person interview with the first author of the paper.

| S-EPMC8182411 | biostudies-literature

miRNA profiling of human plucked hair follicle

Project description:miRNA profiling of human plucked hair follicle from frontal and occipital scalp

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mRNA profiling of human plucked hair follicle

Project description:mRNA profiling of human plucked hair follicle from frontal and occipital scalp

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Autophagy induces hair follicle stem cell activation and hair follicle regeneration by regulating glycolysis

Project description:In this study, in order to explore the role of autophagy of human hair follicle stem cells in hair growth, we explored new ideas for hair regrowth. In this study, rapamycin was used to treat hair follicle stem cells to promote autophagy, and the different expression of genes was observed by comparing with the blank control group.

2024-01-10 | GSE229890 | GEO

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Project description:The homing of exogenous hair follicle mesenchymal stem cells into hair follicle niches

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Hair follicle stem cell gene profile

Project description:Mouse keratinocytes were isolated from K15-EGFP transgenic mice for FACS sorting. RNA samples from EGFP-high and alpha-6 integrin positive cells (hair follicle stem cells) and from EGFP negative and alpha-6 integrin positive cells were used for Microarray analysis. Keywords = stem cells Keywords = hair follicle Keywords = epidermis Keywords = alopecia Keywords: ordered

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