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Regulation of downstream pathway of NSUN2 knockdown in hypopharyngeal carcinoma FaDu cell line

ABSTRACT: Regulation of downstream pathway of NSUN2 knockdown in hypopharyngeal carcinoma FaDu cell line

PROVIDER: PRJNA692368 | ENA |

REPOSITORIES: ENA

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Regulation of downstream pathway of NSUN2 knockdown in hypopharyngeal carcinoma FaDu cell line

Project description:It has been confirmed that nsun2 promotes cell proliferation and migration in hypopharyngeal cancer cell lines, but the pathway of nsun2 in hypopharyngeal cancer cell lines is poorly understood. We constructed stable cell lines by knocking down nsun2 to find the key genes of downstream pathway, and compared them with normal FaDu cell lines.It has been confirmed that nsun2 promotes cell proliferation and migration in FaDu cell line, but the pathway of nsun2 in FaDu cell line is poorly understood. We constructed stable cell lines by knocking down nsun2 to find the key genes of downstream pathway, compared them with normal FaDu cell lines.

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Analysis of differentially expressed lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

Project description:Cisplatin is a common chemotherapeutic drug for hypopharyngeal cancer. But cisplatin-resistance of hypopharyngeal cancer is rarely explored. We cultured hypopharyngeal cancer cell (FaDu) and induced its cisplatin-resistant cell (FaDu/DDP4). The resistance index (RI) of FaDu/DDP4 was 2.828. Then we tested the differentially expressed genes (DEGs) between FaDu and FaDu/DDP4. DEGs contain 2388 lncRNAs, 1932 circRNAs, 745 mRNAs and 202 miRNAs. We used Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzed the DEGs. The differentially expressed 745 mRNAs were classified into 3 domains and 47 secondary GO terms. In KEGG pathway enrichment, “TNF signaling pathway”, “IL-17 signaling pathway” and “JAK-STAT signaling pathway” have greater enrich factors. And we drew the ceRNA networks of DEGs. 52 lncRNAs, 148 circRNAs, 155 mRNAs and 18 miRNAs were selected to draw the network. We noticed several potential targets (as miR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1). At last, we chose 8 miRNAs and 6 mRNAs for qRT-PCR to verify our microarray. In them, miR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1 might be potential genes inducing resistance.

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Project description:To explore the mechanisms underlying the radioresistance of hypopharyngeal carcinoma, we first established specifically radioresistant FaDu cell line (FaDu-RR cells) derived from FaDu cell lines by repeatedly exposing to different doses of ionizing radiation. Then, the aberrantly expressed mRNAs and IncRNAs were detected using microarrays and their bioinformatics were analyzed.

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Transcriptome drift of FADU after expression of HIF-1a, Twist1 or Bmi1

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Transcriptome drift of FADU after expression of HIF-1a, Twist1 or Bmi1

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