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Localized EMT reprograms glial progenitors to promote spinal cord repair [single-cell RNA-seq]

ABSTRACT: Localized EMT reprograms glial progenitors to promote spinal cord repair [single-cell RNA-seq]

PROVIDER: PRJNA692494 | ENA |

REPOSITORIES: ENA

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	SRR13447021.fastq.gz	Fastqsanger.gz

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Localized EMT reprograms glial progenitors to promote spinal cord repair

Project description:Localized EMT reprograms glial progenitors to promote spinal cord repair

| PRJNA692492 | ENA

Localized EMT reprograms glial progenitors to promote spinal cord repair [bulk RNA-seq]

Project description:Localized EMT reprograms glial progenitors to promote spinal cord repair [bulk RNA-seq]

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Localized EMT reprograms glial progenitors to promote spinal cord repair.

Project description:Anti-regenerative scarring obstructs spinal cord repair in mammals and presents a major hurdle for regenerative medicine. In contrast, adult zebrafish possess specialized glial cells that spontaneously repair spinal cord injuries by forming a pro-regenerative bridge across the severed tissue. To identify the mechanisms that regulate differential regenerative capacity between mammals and zebrafish, we first defined the molecular identity of zebrafish bridging glia and then performed cross-species comparisons with mammalian glia. Our transcriptomics show that pro-regenerative zebrafish glia activate an epithelial-to-mesenchymal transition (EMT) gene program and that EMT gene expression is a major factor distinguishing mammalian and zebrafish glia. Functionally, we found that localized niches of glial progenitors undergo EMT after spinal cord injury in zebrafish and, using large-scale CRISPR-Cas9 mutagenesis, we identified the gene regulatory network that activates EMT and drives functional regeneration. Thus, non-regenerative mammalian glia lack an essential EMT-driving gene regulatory network that reprograms pro-regenerative zebrafish glia after injury.

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Localized EMT reprograms glial progenitors to promote spinal cord repair

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-01-31 | GSE164945 | GEO

Localized EMT reprograms glial progenitors to promote spinal cord repair [bulk RNA-seq]

Project description:mCherry/EGFP double positive cells were isolated from the spinal cords of Tg(ctgfa:mCherry; gfap:EGFP) zebrafish at 5 days post injury. Bulk spinal cord tissue at 5, 10, and 21 days post-injury were also sequenced.

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Localized EMT reprograms glial progenitors to promote spinal cord repair [single-cell RNA-seq]

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Project description:Glial cells are present throughout the entire nervous system and paly a crucial role in regulating physiological and pathological functions, such as infections, acute injuries and chronic neurodegenerative disorders. The glial cells mainly include astrocytes, microglia, and oligodendrocytes in the central nervous system (CNS), and satellite glial cells (SGCs) in the peripheral nervous system (PNS). Although the glial subtypes and functional heterogeneity is relatively well understood in mice by recent studies using single-cell or single-nucleus RNA-sequencing, no evidence yet has elucidate the transcriptomic profiles of glia cells in PNS and CNS. Here, we used high-throughput single-nucleus RNA-sequencing to map the cellular and functional heterogeneity of SGCs in human dorsal root ganglion (DRG), and astrocytes, microglia, and oligodendrocytes in human spinal cord. In addition, we compared the human findings with previous single-nucleus transcriptomic profiles of glial cells from mouse DRG and spinal cord. This work will comprehensively profile glial cells heterogeneity and will provide a powerful resource for probing the cellular basis of human physiological and pathological conditions related to glial cells.

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